Hyperoxic and hyperbaric-induced cardioprotection : Role of nitric oxide synthase 3

The relative contributions of the fraction of inspired oxygen (FIO2) and atmospheric pressure (ATM) to cardioprotection are unknown. We determined whether the product of FIO2 x ATM (oxygen partial pressure) controls the extent of hyperoxic+hyperbaric-induced cardioprotection and involves activation...

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Bibliographic Details
Published in:Cardiovascular research 2006-10, Vol.72 (1), p.143-151
Main Authors: CABIGAS, Bernadette P, JIDONG SU, HUTCHINS, William, YANG SHI, SCHAEFER, Richard B, RECINOS, René F, NILAKANTAN, Vani, KINDWALL, Eric, NIEZGODA, Jeffrey A, BAKER, John E
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cardiology. Vascular system
Coronary heart disease
Enzyme Activation
Heart
Heme Oxygenase-1 - metabolism
HSP90 Heat-Shock Proteins - metabolism
Hyperbaric Oxygenation
Male
Medical sciences
Myocardial Ischemia - metabolism
Myocardial Reperfusion
Myocardial Reperfusion Injury - metabolism
Myocardial Reperfusion Injury - prevention & control
Myocardium - chemistry
Nitrates - analysis
Nitric Oxide - metabolism
Nitric Oxide Synthase Type III - metabolism
Oxygen - metabolism
Perfusion
Rats
Rats, Sprague-Dawley
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Summary:The relative contributions of the fraction of inspired oxygen (FIO2) and atmospheric pressure (ATM) to cardioprotection are unknown. We determined whether the product of FIO2 x ATM (oxygen partial pressure) controls the extent of hyperoxic+hyperbaric-induced cardioprotection and involves activation of nitric oxide synthase (NOS). Adult Sprague Dawley rats (n = 10/gp) were treated for 1 h with (1) normoxia+normobaria (21% O2 at 1 ATM), (2) hyperoxia+normobaria (100% O2 at 1 ATM), (3) normoxia+hyperbaria (21% O2 at 2 ATM) and (4) hyperoxia+hyperbaria (100% O2 at 2 ATM). Infarct size following 25 min ischemia and 180 min reperfusion was decreased following hyperoxia+normobaria and normoxia+hyperbaria compared with normoxia+normobaria and further decreased following hyperoxia+hyperbaria treatment. l-NAME (200 microM) reversed the cardioprotective effects of hyperoxia+hyperbaria. Nitrite plus nitrate content was increased 2.2-fold in rats treated with normoxia+hyperbaria and hyperoxia+hyperbaria. NOS3 protein increased 1.2-fold and association of hsp90 with NOS3 four-fold in hyperoxic+hyperbaric rats. Cardioprotection conferred by hyperoxia+hyperbaria is directly dependent on oxygen availability and mediated by NOS.
ISSN:0008-6363
1755-3245
DOI:10.1016/j.cardiores.2006.06.031