Isolation of equine herpesvirus-2 from the lung of an aborted fetus

This study describes the isolation of equine herpesvirus-2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti-EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclona...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2005-09, Vol.17 (5), p.500-502
Main Authors: Galosi, C.M, Cid de la Paz, V, Fernandez, L.C, Martinez, J.P, Craig, M.I, Barrandeguy, M, Etcheverrigaray, M.E
Format: Article
Language:English
Subjects:
Aborted Fetus - virology
abortion (animals)
animal tissues
Animals
Deoxyribonuclease BamHI - metabolism
disease diagnosis
DNA Fingerprinting - veterinary
DNA, Viral - chemistry
DNA, Viral - genetics
equine herpesvirus-2
Female
fetus
Fluorescent Antibody Technique, Indirect - veterinary
Herpesviridae
Herpesviridae Infections - veterinary
Herpesviridae Infections - virology
horse diseases
Horse Diseases - virology
Horses
Lung - embryology
Lung - virology
lungs
Neutralization Tests - veterinary
Polymerase Chain Reaction - veterinary
Pregnancy
Rhadinovirus - genetics
Rhadinovirus - isolation & purification
tissue analysis
Tumor Virus Infections - veterinary
Tumor Virus Infections - virology
viral diseases of animals and humans
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Summary:This study describes the isolation of equine herpesvirus-2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti-EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclonal antibody against EHV-2. Restriction endonuclease DNA fingerprinting with BamHI also confirmed the identity of the virus as EHV-2. Furthermore, viral nucleic acid was detected by polymerase chain reaction from the original lung sample and from the DNA obtained from cells infected with the virus isolate. This work constitutes the first reported isolation of EHV-2 from an aborted equine fetus. The presence of EHV-2 in the lung of the aborted fetus would indicate that this virus is capable of crossing the placental barrier. However, no cause-effect relationship was established between the EHV-2 isolate and the abortion.
ISSN:1040-6387
1943-4936
DOI:10.1177/104063870501700520