Monitoring individual morphological changes over time in ovariectomized rats by in vivo micro-computed tomography

The ovariectomized (OVX) rat is a well established model for osteoporosis research. The recent development of in vivo micro-computed tomography (micro-CT) provides new possibilities to monitor individual bone changes over time. The purpose of this study was to establish the normal time course of bon...

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Bibliographic Details
Published in:Bone (New York, N.Y.) N.Y.), 2006-10, Vol.39 (4), p.854-862
Main Authors: Boyd, Steven K., Davison, Peter, Müller, Ralph, Gasser, Jürg A.
Format: Article
Language:English
Subjects:
Age Factors
Analysis of Variance
Animals
Biological and medical sciences
Bone and Bones - pathology
Bone histomorphometry
Disease Models, Animal
Diseases of the osteoarticular system
Female
Fundamental and applied biological sciences. Psychology
Histomorphometry
Medical sciences
Micro-computed tomography
Osteopenia
Osteoporosis
Osteoporosis - etiology
Osteoporosis - pathology
Osteoporosis. Osteomalacia. Paget disease
Ovariectomy - adverse effects
Random Allocation
Rat
Rats
Rats, Wistar
Tibial metaphyses
Tomography, X-Ray Computed - methods
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:The ovariectomized (OVX) rat is a well established model for osteoporosis research. The recent development of in vivo micro-computed tomography (micro-CT) provides new possibilities to monitor individual bone changes over time. The purpose of this study was to establish the normal time course of bone loss in the OVX rat model, and to determine the ability to detect morphological changes in vivo compared to cross-sectional study designs where animals are sacrificed at each time point. Eight-month-old female Wistar rats were randomly assigned to one of two groups: OVX ( N = 10) or sham-operated ( N = 10). In vivo micro-CT scanning of the right proximal tibial metaphyses occurred at 1-month intervals for 6 months. Morphological analyses were performed at each time step for every animal, and a two-way ANOVA with repeated measures was used to analyze the data. A second statistical analysis was performed without repeated measures for analysis as a cross-sectional study design. The repeated measures analysis was more sensitive to early changes than the cross-sectional study analysis. Changes were detected by longitudinal analysis in the sham-operated and OVX animals over time ( P 
ISSN:8756-3282
1873-2763
DOI:10.1016/j.bone.2006.04.017