Invasive phenotype and apoptosis induction of Plesiomonas shigelloides P‐1 strain to Caco‐2 cells

Aims:  The mechanism of the host cell invasion of Plesiomonas shigelloides and its capability to induce apoptosis were investigated. Methods and Results:  We performed a time course experiment on the bacterial adherence and invasion of the P. shigelloides P‐1 strain into Caco‐2 cells using an invasi...

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Bibliographic Details
Published in:Journal of applied microbiology 2005-01, Vol.99 (6), p.1435-1443
Main Authors: Tsugawa, H., Ono, T., Murakami, H., Okawa, Y.
Format: Article
Language:English
Subjects:
Apoptosis
Bacterial Adhesion
Bacteriological Techniques
Biological and medical sciences
Caco-2 Cells - microbiology
Caco-2 Cells - pathology
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Gram-Negative Bacterial Infections - microbiology
Gram-Negative Bacterial Infections - transmission
Humans
In Situ Nick-End Labeling
invasion
Microbiology
Plesiomonas - pathogenicity
Plesiomonas shigelloides
Time Factors
Virulence
virulence factor
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Summary:Aims:  The mechanism of the host cell invasion of Plesiomonas shigelloides and its capability to induce apoptosis were investigated. Methods and Results:  We performed a time course experiment on the bacterial adherence and invasion of the P. shigelloides P‐1 strain into Caco‐2 cells using an invasion assay and flow cytometry. The adherence of P. shigelloides to the Caco‐2 cells was almost completed within 10 min after the infection. Thereafter, P. shigelloides starts internalization within the Caco‐2 cells, which was completed within 60 min after the infection. Based on the invasion assay using nocodazole, cytochalasin D, and genistein, it became clear that the mechanism of the internalization depended on the signal transduction followed by the rearrangement of the cytoskeletal protein. Based on the DNA laddering and TUNEL methods, the cytotoxicity of the Caco‐2 cells by the invasion of P. shigelloides occurred through the induction of apoptosis. Conclusions:  This work demonstrated that the mechanism of invasion of P. shigelloides into Caco‐2 cells and the invasion of P. shigelloides induces apoptotic cell death. Significance and Impact of the Study:  This work revealed the virulence factor, which may be important for understanding of the pathogenesis of P. shigelloides.
ISSN:1364-5072
1365-2672
DOI:10.1111/j.1365-2672.2005.02721.x