Suppression of apolipoprotein B secretion from HepG2 cells by glucosyl hesperidin

• Published in: Journal of Nutritional Science and Vitaminology 2006, Vol.52(3), pp.223-231
• Main Authors: Miwa, Y.(Hayashibara Biochemical Labs. Inc., Okayama (Japan)), Mitsuzumi, H, Yamada, M, Arai, N, Tanabe, F, Okada, K, Kubota, M, Chaen, H, Sunayama, T, Kibata, M
• Format: Article
• Language: English
• Subjects: Analysis of Variance; apolipoprotein B; Apolipoproteins B - metabolism; Biological and medical sciences; Carcinoma, Hepatocellular - metabolism; Cells, Cultured; CHOLESTEROL; Cholesterol Esters - metabolism; cholesteryl ester; COLESTEROL; Enzyme-Linked Immunosorbent Assay - methods; Feeding. Feeding behavior; FLAVONOIDE; FLAVONOIDES; FLAVONOIDS; Fundamental and applied biological sciences. Psychology; Glucosides - chemistry; Glucosides - pharmacology; glucosyl hesperidin; HepG2 cells; Hesperidin - analogs & derivatives; Hesperidin - chemistry; Hesperidin - pharmacology; Humans; In Vitro Techniques; LIPOPROTEINAS; LIPOPROTEINE; LIPOPROTEINS; Lipoproteins, VLDL - metabolism; Liver Neoplasms - metabolism; Models, Biological; Time Factors; TRIGLICERIDOS; TRIGLYCERIDE; TRIGLYCERIDES; Triglycerides - metabolism; Vertebrates: anatomy and physiology, studies on body, several organs or systems; very low-density lipoprotein
• ISSN: 0301-4800; 1881-7742
• DOI: 10.3177/jnsv.52.223

Our previous study has shown that a soluble hesperidin derivative, glucosyl hesperidin (G-hesperidin), preferentially lowers serum triglyceride (TG) level in hypertriglyceridemic subjects through the improvement of very low-density lipoprotein (VLDL) metabolic abnormality. G-Hesperidin has also been found to decrease an elevated serum apolipoprotein B (apo B) level in the hypertriglyceridemic subjects, suggesting a possibility that this compound suppresses excess VLDL secretion in the liver. In the present study, to gain a better understanding of possible mechanisms by which G-hesperidin lowers serum TG, we examined whether this derivative affects apo B secretion from HepG2 human hepatoma cells, a model of hepatic VLDL secretion. As a result, G-hesperidin significantly reduced apo B secretion from the oleate-stimulated HepG2 cells. Furthermore, G-hesperidin significantly suppressed apo B secretion only in the oleate-stimulated cells and failed to act on the cells incubated without oleate. In the oleate-stimulated cells, G-hesperidin significantly decreased cellular cholesteryl ester (CE), although it had no effect on cellular TG or free cholesterol amounts. Moreover, the oleate-stimulated cells had a decrease in cellular apo B amounts by G-hesperidin exposure. These findings indicate that G-hesperidin downregulates the assembly of apo B-containing lipoproteins via the reduction of CE synthesis augmented with oleate and results in suppressing excess apo B secretion from the cells. This effect is speculated to be associated with the improvement of VLDL metabolic abnormality in hypertriglyceridemic subjects and considered as a mechanism of lowering serum TG.