Simultaneous Detection of Creatine and Creatinine using a Sequential Injection Analysis/Biosensor System

Carbon paste based biosensors for the determination of creatine and creatinine have been integrated into a sequential injection system. Applying the multi-enzyme sequence of creatininase (CA), and/or creatinase (CI) and sarcosine oxidase (SO), hydrogen peroxide has been detected amperometrically. Th...

Full description

Saved in:
Bibliographic Details
Published in:Preparative biochemistry & biotechnology 2006-12, Vol.36 (4), p.287-296
Main Authors: Stefan-van Staden, Raluca-Ioana, Bokretsion, Rahel Girmai, van Staden, Jacobus F., Aboul-Enein, Hassan Y.
Format: Article
Language:English
Subjects:
Amidohydrolases - chemistry
Amperometric biosensors
Biosensing Techniques - instrumentation
Carbon - chemistry
Creatinase
Creatine
Creatine - analysis
Creatine - blood
Creatininase
Creatinine
Creatinine - analysis
Creatinine - blood
Enzymes, Immobilized - chemistry
Flow Injection Analysis - instrumentation
Hydrogen Peroxide - chemistry
Online Systems - instrumentation
Sarcosine oxidase
Sarcosine Oxidase - chemistry
Sequential injection analysis
Ureohydrolases - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Carbon paste based biosensors for the determination of creatine and creatinine have been integrated into a sequential injection system. Applying the multi-enzyme sequence of creatininase (CA), and/or creatinase (CI) and sarcosine oxidase (SO), hydrogen peroxide has been detected amperometrically. The linear concentration ranges are of pmol/L to nmol/L magnitude, with very low limits of detection. The proposed SIA system can be utilized reliably for the on-line simultaneous detection of creatine and creatinine in pharmaceutical products, as well as in serum samples, with a rate of 34 samples per hour and RSD values better than 0.16% (n=10).
ISSN:1082-6068
1532-2297
DOI:10.1080/10826060600912393