Proportion of antibiotic resistance in subgingival plaque samples from Mexican subjects

• Published in: Journal of clinical periodontology 2006-10, Vol.33 (10), p.743-748
• Main Authors: Almaguer-Flores, Argelia, Moreno-Borjas, Jazmin-Yunuen, Salgado-Martinez, Araceli, Sanchez-Reyes, Marco-Antonio, Alcantara-Maruri, Eulalio, Ximenez-Fyvie, Laurie-Ann
• Format: Article
• Language: English
• Subjects: Adult; Amoxicillin - pharmacology; Anti-Bacterial Agents - pharmacology; antibiotic resistance; Bacteria - classification; Bacteria - drug effects; Bacteriological Techniques; Colony Count, Microbial; Dental Plaque - microbiology; Dentistry; Doxycycline - pharmacology; Drug Resistance, Bacterial; Female; Gram-Negative Bacteria - classification; Gram-Negative Bacteria - drug effects; Humans; Male; Mexican subjects; Mexico; Microbial Sensitivity Tests; Middle Aged; Penicillin Resistance; periodontal disease; subgingival plaque; Tetracycline Resistance
• ISSN: 0303-6979; 1600-051X
• DOI: 10.1111/j.1600-051X.2006.00975.x

Aim: To determine the proportion of bacteria resistant to amoxicillin and doxycycline in subgingival plaque samples from Mexican subjects. Materials and Methods: Two subgingival plaque samples were taken from 20 Mexican subjects. Samples were dispersed, diluted and plated on non‐antibiotic agar plates and on plates containing 0.5, 1, 2, 4, 8 and 16 μg/ml of either amoxicillin or doxycycline. The proportion of resistant bacteria was calculated based on the total number of colony‐forming units present in the non‐antibiotic containing plates. Results: On average, 0.4–13.4% and 0.9–20.4% of the total cultivable subgingival microbiota was resistant to the concentrations tested of amoxicillin and doxycycline, respectively. The differences between antibiotics were statistically significant for the 0.5, 2 and 4 μg/ml concentrations (p