EGF and FGF-2 responsiveness of rat and mouse neural precursors derived from the embryonic CNS

EGF and FGF-2 induce the proliferation of embryonic neural precursors (ENPs) in vitro from a number of different species. In this study, we demonstrate that embryonic age is a crucial determinant of the number and differentiation potential of rat embryonic neural precursor cells responding to either...

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Bibliographic Details
Published in:Brain research bulletin 2005-12, Vol.68 (1), p.83-94
Main Authors: Kelly, Claire M., Tyers, Pam, Borg, Melanie ter, Svendsen, Clive N., Dunnett, Stephen B., Rosser, Anne E.
Format: Article
Language:English
Subjects:
Animals
Anticoagulants - pharmacology
Brain Stem - cytology
Cell Culture Techniques - methods
Cell Differentiation - drug effects
Cell Division - drug effects
Corpus Striatum - cytology
EGF
Epidermal Growth Factor - pharmacology
Female
Fetus - cytology
FGF-2
Fibroblast Growth Factor 2 - pharmacology
Gestational Age
Heparin - pharmacology
Mice
Neuronal differentiation
Neurons - cytology
Pregnancy
Proliferation
Rats
Stem Cells - cytology
Stem Cells - drug effects
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Summary:EGF and FGF-2 induce the proliferation of embryonic neural precursors (ENPs) in vitro from a number of different species. In this study, we demonstrate that embryonic age is a crucial determinant of the number and differentiation potential of rat embryonic neural precursor cells responding to either EGF and/or FGF-2, in that (i) there is a differential response to the two growth factors (both alone and in combination) according to the gestational age of isolation and (ii) when allowed to differentiate, there are temporal changes in the ability of these cells to produce neurons. Furthermore, for cultures of all gestational ages, there is a defined pattern of senescence, with cultures expanding longest when cells are isolated earlier in gestation. The suggestion is that rat ENPs in this study consist predominantly of neural progenitor cells with limited division potential rather than self-renewing multipotential neural stem cells. In contrast, mouse ENPs appeared to expand indefinitely and thus allow for longer studies to be carried out looking at the effects of growth factor concentrations. The effect of varying the concentration of EGF was assessed using mouse ENPs.
ISSN:0361-9230
1873-2747
DOI:10.1016/j.brainresbull.2005.08.020