Comparative cDNA-AFLP analysis of Cd-tolerant and -sensitive genotypes derived from crosses between the Cd hyperaccumulator Arabidopsis halleri and Arabidopsis lyrata ssp. petraea

• Published in: Journal of experimental botany 2006-09, Vol.57 (12), p.2967-2983
• Main Authors: Craciun, Adrian Radu, Courbot, Mikael, Bourgis, Fabienne, Salis, Pietrino, Saumitou-Laprade, Pierre, Verbruggen, Nathalie
• Format: Article
• Language: English
• Subjects: amplified fragment length polymorphism; Arabidopsis; Arabidopsis - drug effects; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis halleri; Arabidopsis lyrata subsp. petraea; backcrossing; Biological and medical sciences; cadmium; Cadmium - metabolism; Cadmium - pharmacology; cDNA-AFLP; complementary DNA; Crosses, Genetic; culture media; DNA, Complementary - chemistry; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Profiling; gene expression regulation; Gene Expression Regulation, Plant - drug effects; gene segregation; genes; Genes. Genome; Genotype; heavy metal tolerance; Hydroponics; hyperaccumulator; hyperaccumulators; interspecific hybridization; metal tolerance; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; phytotoxicity; plant proteins; Plant Roots - drug effects; Plant Roots - genetics; Plant Roots - metabolism; Polymorphism, Genetic; Reverse Transcriptase Polymerase Chain Reaction; roots; Sequence Analysis, DNA; Signal Transduction - drug effects; stress tolerance mechanisms; transcriptome; transcriptomics; wild plants
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/erl062

Cadmium (Cd) tolerance seems to be a constitutive species-level trait in Arabidopsis halleri. In order to identify genes potentially implicated in Cd tolerance, a backcross (BC1) segregating population was produced from crosses between A. halleri ssp. halleri and its closest non-tolerant relative A. lyrata ssp. petraea. The most sensitive and tolerant genotypes of the BC1 were analysed on a transcriptome-wide scale by cDNA-amplified fragment length polymorphism (AFLP). A hundred and thirty-four genes expressed more in the root of tolerant genotypes than in sensitive genotypes were identified. Most of the identified genes showed no regulation in their expression when exposed to Cd in a hydroponic culture medium and belonged to diverse functional classes, including reactive oxygen species (ROS) detoxification, cellular repair, metal sequestration, water transport, signal transduction, transcription regulation, and protein degradation, which are discussed.