Noninvasive prenatal RHD genotyping by real-time polymerase chain reaction using plasma from D-negative pregnant women

Prenatal noninvasive determination of fetal Rh status is an important aid to the management of hemolytic disease of the fetus and newborn. We performed real-time polymerase chain reaction on fetal DNA derived from maternal plasma to determine fetal Rh status. Cell-free plasma DNA from 98 D-negative...

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Bibliographic Details
Published in:American journal of obstetrics and gynecology 2005-12, Vol.193 (6), p.1966-1971
Main Authors: Zhou, Lan, Thorson, John A., Nugent, Clark, Davenport, Robertson D., Butch, Suzanne H., Judd, W. John
Format: Article
Language:English
Subjects:
Algorithms
Biological and medical sciences
Cell-Free System
Female
Fetal Blood
Fetal DNA in maternal plasma
Genotype
Gynecology. Andrology. Obstetrics
Humans
Infant, Newborn
Male
Maternal-Fetal Exchange
Medical sciences
Polymerase Chain Reaction - methods
Pregnancy
Prenatal RHD genotyping
Real-time polymerase chain reaction
Rh-Hr Blood-Group System - blood
Sex-Determining Region Y Protein - blood
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Summary:Prenatal noninvasive determination of fetal Rh status is an important aid to the management of hemolytic disease of the fetus and newborn. We performed real-time polymerase chain reaction on fetal DNA derived from maternal plasma to determine fetal Rh status. Cell-free plasma DNA from 98 D-negative pregnant women was tested for the presence of exons 4, 5, and 10 of RHD. The presence of fetal DNA was confirmed by detection of SRY or biallelic insertion/deletion polymorphisms in the maternal plasma and buffy coat. Seventy-two D-positive infants and 26 D-negative infants were determined by serologic studies. All 3 RHD exon sequences were detected in 68 of 72 mothers of D-positive infants. The presence of fetal DNA in mothers of D-negative infants was confirmed in all 10 boys and in 14 of 16 girls. Fetal RHD genotyping in this study correctly predicted fetal Rh status in 92 of 98 (94%) cases.
ISSN:0002-9378
1097-6868
DOI:10.1016/j.ajog.2005.04.052