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Noninvasive prenatal RHD genotyping by real-time polymerase chain reaction using plasma from D-negative pregnant women
Prenatal noninvasive determination of fetal Rh status is an important aid to the management of hemolytic disease of the fetus and newborn. We performed real-time polymerase chain reaction on fetal DNA derived from maternal plasma to determine fetal Rh status. Cell-free plasma DNA from 98 D-negative...
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| Published in: | American journal of obstetrics and gynecology 2005-12, Vol.193 (6), p.1966-1971 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c384t-9233534bd6d0ec2eb7b18a5e34955c132efd3412f65dd5f67d9576cf4a66b8a83 |
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| cites | cdi_FETCH-LOGICAL-c384t-9233534bd6d0ec2eb7b18a5e34955c132efd3412f65dd5f67d9576cf4a66b8a83 |
| container_end_page | 1971 |
| container_issue | 6 |
| container_start_page | 1966 |
| container_title | American journal of obstetrics and gynecology |
| container_volume | 193 |
| creator | Zhou, Lan Thorson, John A. Nugent, Clark Davenport, Robertson D. Butch, Suzanne H. Judd, W. John |
| description | Prenatal noninvasive determination of fetal Rh status is an important aid to the management of hemolytic disease of the fetus and newborn. We performed real-time polymerase chain reaction on fetal DNA derived from maternal plasma to determine fetal Rh status.
Cell-free plasma DNA from 98 D-negative pregnant women was tested for the presence of exons 4, 5, and 10 of
RHD. The presence of fetal DNA was confirmed by detection of
SRY or biallelic insertion/deletion polymorphisms in the maternal plasma and buffy coat.
Seventy-two D-positive infants and 26 D-negative infants were determined by serologic studies. All 3
RHD exon sequences were detected in 68 of 72 mothers of D-positive infants. The presence of fetal DNA in mothers of D-negative infants was confirmed in all 10 boys and in 14 of 16 girls.
Fetal
RHD genotyping in this study correctly predicted fetal Rh status in 92 of 98 (94%) cases. |
| doi_str_mv | 10.1016/j.ajog.2005.04.052 |
| format | article |
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Cell-free plasma DNA from 98 D-negative pregnant women was tested for the presence of exons 4, 5, and 10 of
RHD. The presence of fetal DNA was confirmed by detection of
SRY or biallelic insertion/deletion polymorphisms in the maternal plasma and buffy coat.
Seventy-two D-positive infants and 26 D-negative infants were determined by serologic studies. All 3
RHD exon sequences were detected in 68 of 72 mothers of D-positive infants. The presence of fetal DNA in mothers of D-negative infants was confirmed in all 10 boys and in 14 of 16 girls.
Fetal
RHD genotyping in this study correctly predicted fetal Rh status in 92 of 98 (94%) cases.</description><identifier>ISSN: 0002-9378</identifier><identifier>EISSN: 1097-6868</identifier><identifier>DOI: 10.1016/j.ajog.2005.04.052</identifier><identifier>PMID: 16325598</identifier><identifier>CODEN: AJOGAH</identifier><language>eng</language><publisher>Philadelphia, PA: Mosby, Inc</publisher><subject>Algorithms ; Biological and medical sciences ; Cell-Free System ; Female ; Fetal Blood ; Fetal DNA in maternal plasma ; Genotype ; Gynecology. Andrology. Obstetrics ; Humans ; Infant, Newborn ; Male ; Maternal-Fetal Exchange ; Medical sciences ; Polymerase Chain Reaction - methods ; Pregnancy ; Prenatal RHD genotyping ; Real-time polymerase chain reaction ; Rh-Hr Blood-Group System - blood ; Sex-Determining Region Y Protein - blood</subject><ispartof>American journal of obstetrics and gynecology, 2005-12, Vol.193 (6), p.1966-1971</ispartof><rights>2005 Mosby, Inc.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-9233534bd6d0ec2eb7b18a5e34955c132efd3412f65dd5f67d9576cf4a66b8a83</citedby><cites>FETCH-LOGICAL-c384t-9233534bd6d0ec2eb7b18a5e34955c132efd3412f65dd5f67d9576cf4a66b8a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17333897$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16325598$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Lan</creatorcontrib><creatorcontrib>Thorson, John A.</creatorcontrib><creatorcontrib>Nugent, Clark</creatorcontrib><creatorcontrib>Davenport, Robertson D.</creatorcontrib><creatorcontrib>Butch, Suzanne H.</creatorcontrib><creatorcontrib>Judd, W. John</creatorcontrib><title>Noninvasive prenatal RHD genotyping by real-time polymerase chain reaction using plasma from D-negative pregnant women</title><title>American journal of obstetrics and gynecology</title><addtitle>Am J Obstet Gynecol</addtitle><description>Prenatal noninvasive determination of fetal Rh status is an important aid to the management of hemolytic disease of the fetus and newborn. We performed real-time polymerase chain reaction on fetal DNA derived from maternal plasma to determine fetal Rh status.
Cell-free plasma DNA from 98 D-negative pregnant women was tested for the presence of exons 4, 5, and 10 of
RHD. The presence of fetal DNA was confirmed by detection of
SRY or biallelic insertion/deletion polymorphisms in the maternal plasma and buffy coat.
Seventy-two D-positive infants and 26 D-negative infants were determined by serologic studies. All 3
RHD exon sequences were detected in 68 of 72 mothers of D-positive infants. The presence of fetal DNA in mothers of D-negative infants was confirmed in all 10 boys and in 14 of 16 girls.
Fetal
RHD genotyping in this study correctly predicted fetal Rh status in 92 of 98 (94%) cases.</description><subject>Algorithms</subject><subject>Biological and medical sciences</subject><subject>Cell-Free System</subject><subject>Female</subject><subject>Fetal Blood</subject><subject>Fetal DNA in maternal plasma</subject><subject>Genotype</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Male</subject><subject>Maternal-Fetal Exchange</subject><subject>Medical sciences</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Pregnancy</subject><subject>Prenatal RHD genotyping</subject><subject>Real-time polymerase chain reaction</subject><subject>Rh-Hr Blood-Group System - blood</subject><subject>Sex-Determining Region Y Protein - blood</subject><issn>0002-9378</issn><issn>1097-6868</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNp9kE2L1EAQhhtR3NnVP-BB-qK3jP2R7nTAi-zqrrAoiJ6bSqcSe0i6Y3dmZP69CRPYm6eiqOd9KR5C3nC254zrD4c9HGK_F4ypPSv3TIlnZMdZXRXaaPOc7BhjoqhlZa7Idc6HdRW1eEmuuJZCqdrsyOlbDD6cIPsT0ilhgBkG-uPhjvYY4nyefOhpc6YJYShmPy5QHM4jJshI3W_wYT252cdAj3mFpwHyCLRLcaR3RcAe5q27DxBm-jeOGF6RFx0MGV9v84b8-vL55-1D8fj9_uvtp8fCSVPORS2kVLJsWt0ydAKbquEGFMqyVspxKbBrZclFp1Xbqk5Xba0q7boStG4MGHlD3l96pxT_HDHPdvTZ4TBAwHjMVhujuarZAooL6FLMOWFnp-RHSGfLmV1t24NdbdvVtmWlXWwvobdb-7EZsX2KbHoX4N0GQHYwdAmC8_mJq6SUpq4W7uOFw8XFyWOy2XkMDluf0M22jf5_f_wDG22fmQ</recordid><startdate>20051201</startdate><enddate>20051201</enddate><creator>Zhou, Lan</creator><creator>Thorson, John A.</creator><creator>Nugent, Clark</creator><creator>Davenport, Robertson D.</creator><creator>Butch, Suzanne H.</creator><creator>Judd, W. 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John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-9233534bd6d0ec2eb7b18a5e34955c132efd3412f65dd5f67d9576cf4a66b8a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Algorithms</topic><topic>Biological and medical sciences</topic><topic>Cell-Free System</topic><topic>Female</topic><topic>Fetal Blood</topic><topic>Fetal DNA in maternal plasma</topic><topic>Genotype</topic><topic>Gynecology. Andrology. 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John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Noninvasive prenatal RHD genotyping by real-time polymerase chain reaction using plasma from D-negative pregnant women</atitle><jtitle>American journal of obstetrics and gynecology</jtitle><addtitle>Am J Obstet Gynecol</addtitle><date>2005-12-01</date><risdate>2005</risdate><volume>193</volume><issue>6</issue><spage>1966</spage><epage>1971</epage><pages>1966-1971</pages><issn>0002-9378</issn><eissn>1097-6868</eissn><coden>AJOGAH</coden><abstract>Prenatal noninvasive determination of fetal Rh status is an important aid to the management of hemolytic disease of the fetus and newborn. We performed real-time polymerase chain reaction on fetal DNA derived from maternal plasma to determine fetal Rh status.
Cell-free plasma DNA from 98 D-negative pregnant women was tested for the presence of exons 4, 5, and 10 of
RHD. The presence of fetal DNA was confirmed by detection of
SRY or biallelic insertion/deletion polymorphisms in the maternal plasma and buffy coat.
Seventy-two D-positive infants and 26 D-negative infants were determined by serologic studies. All 3
RHD exon sequences were detected in 68 of 72 mothers of D-positive infants. The presence of fetal DNA in mothers of D-negative infants was confirmed in all 10 boys and in 14 of 16 girls.
Fetal
RHD genotyping in this study correctly predicted fetal Rh status in 92 of 98 (94%) cases.</abstract><cop>Philadelphia, PA</cop><pub>Mosby, Inc</pub><pmid>16325598</pmid><doi>10.1016/j.ajog.2005.04.052</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0002-9378 |
| ispartof | American journal of obstetrics and gynecology, 2005-12, Vol.193 (6), p.1966-1971 |
| issn | 0002-9378 1097-6868 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68861590 |
| source | Elsevier |
| subjects | Algorithms Biological and medical sciences Cell-Free System Female Fetal Blood Fetal DNA in maternal plasma Genotype Gynecology. Andrology. Obstetrics Humans Infant, Newborn Male Maternal-Fetal Exchange Medical sciences Polymerase Chain Reaction - methods Pregnancy Prenatal RHD genotyping Real-time polymerase chain reaction Rh-Hr Blood-Group System - blood Sex-Determining Region Y Protein - blood |
| title | Noninvasive prenatal RHD genotyping by real-time polymerase chain reaction using plasma from D-negative pregnant women |
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