Features of ionogenic group composition in polymeric matrix of lily pollen wall

The composition of ionogenic groups and ion-exchange capacity were studied in the polymeric matrix of cell walls isolated from the pollen grain and tissues of vegetative organs (leaves and stems) of Lilium longiflorum Thunb. The ion-exchange capacity was evaluated at different pH values and ionic st...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2006-08, Vol.71 (8), p.893-899
Main Authors: Meychik, N R, Matveyeva, N P, Nikolaeva, Yu I, Chaikova, A V, Yermakov, I P
Format: Article
Language:English
Subjects:
Biopolymers - chemistry
Cell Wall - chemistry
Coumaric Acids - chemistry
Cross-Linking Reagents
Hydrogen-Ion Concentration
Ion exchange
Ionization
Lilium - chemistry
Lilium - ultrastructure
Osmolar Concentration
Phenols
Pollen
Pollen - chemistry
Pollen - ultrastructure
Polymers
Polysaccharides - chemistry
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Summary:The composition of ionogenic groups and ion-exchange capacity were studied in the polymeric matrix of cell walls isolated from the pollen grain and tissues of vegetative organs (leaves and stems) of Lilium longiflorum Thunb. The ion-exchange capacity was evaluated at different pH values and ionic strength of 100 mM. In the two-layered pollen wall and the somatic cell walls four types of ionogenic groups were found: amino groups, two carboxyl groups (represented by residues of uronic and hydroxycinnamic acids), and phenolic OH-groups. The groups of all four types are present in the intine, whereas the exine contains one type of anion-exchange and two types of cation-exchange groups. The contents of each type group and their ionization constants were determined. The qualitative and quantitative compositions of structural polymers of the pollen intine and somatic cell walls are significantly different. It is suggested that hydroxycinnamic acids should be involved in cross-linking of polysaccharide chains in both the intine and somatic cell primary walls, and such cross-links play a crucial role in the structural organization and integrity of the pollen grain wall.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297906080116