Effect of menopause on platelet activation markers determined by flow cytometry

Pre‐menopausal women have a lower risk of cardiovascular disease compared to post‐menopausal women. Cardiovascular disease is more age dependent in women than in men. The association of platelet activation and cardiovascular thrombotic events is well established. Standardized techniques were used to...

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Bibliographic Details
Published in:American journal of hematology 2005-12, Vol.80 (4), p.257-261
Main Authors: Roshan, Tariq M., Normah, Jamalludin, Rehman, Asia, Naing, Lin
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Blood Platelets - metabolism
Dual Specificity Phosphatase 2
Estradiol - blood
Female
Flow Cytometry
Hematologic and hematopoietic diseases
Humans
Integrin beta3 - biosynthesis
Medical sciences
Middle Aged
P-Selectin - biosynthesis
platelet
Platelet Activation
Postmenopause - blood
post‐menopausal women
Premenopause - blood
Protein Phosphatase 2
Protein Tyrosine Phosphatases - biosynthesis
Risk Factors
Thrombosis - blood
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Summary:Pre‐menopausal women have a lower risk of cardiovascular disease compared to post‐menopausal women. Cardiovascular disease is more age dependent in women than in men. The association of platelet activation and cardiovascular thrombotic events is well established. Standardized techniques were used to evaluate platelet activation markers by flow cytometry, using 3‐color analysis (CD 61PerCP, CD 62P, and PAC‐1) in 49 post‐menopausal (mean ± SD age, 56.16 ± 33.51 years) and 42 pre‐menopausal (age, 39.38 ± 7.07 years) women. Results of our study showed a significant increase in CD 62P in post‐menopausal women as compared to the pre‐menopausal group (2.66 ± 4.26% vs. 0.52 ± 2.71%, P < 0.001). Similarly, PAC‐1 was significantly increased in post‐menopausal women (21.54 ± 2.48% vs. 3.70 ± 2.31%, P < 0.001). Furthermore, there was a significant association of CD 62P with serum estradiol in both groups. PAC‐1 was significantly associated with age in both groups. The results suggest the role of platelets in the increased incidence of thrombotic events and disease in post‐menopausal women. Am. J. Hematol. 80:257–261, 2005. © 2005 Wiley‐Liss, Inc.
ISSN:0361-8609
1096-8652
DOI:10.1002/ajh.20472