Capillary electrophoresis-based separation techniques for the analysis of proteins

• Published in: Electrophoresis 2006-09, Vol.27 (18), p.3503-3522
• Main Authors: Huang, Yu-Fen, Huang, Chih-Chin, Hu, Cho-Chun, Chang, Huan-Tsung
• Format: Article
• Language: English
• Subjects: Capillary electrophoresis; Electrophoresis, Capillary - methods; Humans; Mass Spectrometry; Multidimensional separation; Proteins - analysis; Proteins - isolation & purification; Stacking
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/elps.200600100

CE offers the advantages of high speed, great efficiency, as well as the requirement of minimum amounts of sample and buffer for the analysis of proteins. In this review, we summarize the CE‐based techniques coupled with absorption, LIF, and MS detection systems for the analysis of proteins mostly within the past 5 years. The basic principle of each technique and its advantages and disadvantages for protein analysis are discussed in brief. Advanced CE techniques, including on‐column concentration techniques and high‐efficiency multidimensional separation techniques, for high‐throughput protein profiling of complex biological samples and/or of single cells are emphasized. Although the developed techniques provide improved peak capacity, they have not become practical tools for proteomics, mainly because of poor reproducibility, low‐sample lading capacity, and low throughput due to ineffective interfaces between two separation dimensions and that between separation and MS systems. In order to identify the complexities and dynamics of the proteomes expressed by cells, tissues, or organisms, techniques providing improved analytical sensitivity, throughput, and dynamic ranges are still demanded. This paper is dedicated to the memory of a great mentor of H. T. Chang, Andreas Chrambach.