Regulation of gene and protein expression in cardiac myocyte hypertrophy and apoptosis

Considerable efforts have been expended in elucidating the inter-cellular and intra-cellular signaling pathways which elicit cardiac myocyte hypertrophy or apoptosis, and in identifying the changes which are associated with the end-stage of the response. The challenge now is to link the two. Althoug...

Full description

Saved in:
Bibliographic Details
Published in:Advances in enzyme regulation 2005, Vol.45 (1), p.94-111
Main Authors: Clerk, Angela, Cullingford, Timothy E, Kemp, Timothy J, Kennedy, Robert A, Sugden, Peter H
Format: Article
Language:English
Subjects:
Animals
Apoptosis - physiology
Gene Expression Regulation - physiology
Heat-Shock Proteins - biosynthesis
Hypertrophy - physiopathology
MAP Kinase Signaling System - physiology
Myocytes, Cardiac - physiology
Peroxidases - biosynthesis
Peroxiredoxins
Proteomics
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Considerable efforts have been expended in elucidating the inter-cellular and intra-cellular signaling pathways which elicit cardiac myocyte hypertrophy or apoptosis, and in identifying the changes which are associated with the end-stage of the response. The challenge now is to link the two. Although some of the signaling effects will be the acute modulation of existing protein function, long-term effects which bring about and maintain the hypertrophic state or which culminate in cell death are mediated at the level of gene and protein expression. With the advances in micro-array technology and genome sequencing, it is now possible to obtain a picture of the global gene expression profile in myocytes or in whole heart which dictates the proteins which could be made. This is not the final picture since additional regulation at the level of translation modulates the relative proportions of each protein that can be made from the transcriptome. Even here, further regulation of protein stability and turnover means that ultimately it is still necessary to examine the proteome to determine what may cause the functional changes in a cell. Thus, in order to gain a full picture of events which regulate the response and gain some insight into possible points of intervention for therapy, it is necessary to examine gene expression, mRNA translation and protein expression in concert.
ISSN:0065-2571
DOI:10.1016/j.advenzreg.2005.02.007