Increasing the luminescence of lanthanide complexes

• Published in: Cytometry. Part A 2006-08, Vol.69A (8), p.767-778
• Main Authors: Leif, Robert C., Vallarino, Lidia M., Becker, Margie C., Yang, Sean
• Format: Article
• Language: English
• Subjects: Animals; columinescence; europium; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Humans; Image Cytometry - methods; lanthanide; Lanthanoid Series Elements - chemistry; luminescence; macrocycle; Nanostructures; Quantum Dots; Quantum Dye; RETEL; Spectrometry, Fluorescence - methods; terbium; time‐delayed
• ISSN: 1552-4922; 1552-4930
• DOI: 10.1002/cyto.a.20321

This review compares the chemical and physical properties of lanthanide ion complexes and of other narrow‐emitting species that can be used as labels for cytometry. A series of luminescent lanthanide ion macrocyclic complexes, Quantum Dyes®, which do not release or exchange their central lanthanide ion, do accept energy transfer from ligands, and are capable of covalent binding to macromolecules, including proteins and nucleic acids, is described and their properties are discussed. Two methods are described for increasing the luminescence intensity of lanthanide ion complexes, which intrinsically is not as high as that of standard fluorophores or quantum dots. One method consists of adding a complex of a second lanthanide ion in a micellar solution (columinescence); the other method produces dry preparations by evaporation of a homogeneous solution containing an added complex of a second lanthanide ion or an excess of an unbound antenna ligand. Both methods involve the Resonance Energy Transfer Enhanced Luminescence, RETEL, effect as the mechanism for the luminescence enhancement. © 2006 International Society for Analytical Cytology