Genetic Diversity and Recombination of Porcine Sapoviruses

Sapoviruses (SaVs) are emerging enteric pathogens that cause diarrhea in humans and animals. Human SaVs are genetically variable and have been classified into four genogroups (GI, -II, -IV, and -V). To date, only two genetically similar porcine SaV strains have been reported that belong to GIII. To...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2005-12, Vol.43 (12), p.5963-5972
Main Authors: Wang, Qiu-Hong, Han, Myung Guk, Funk, Julie A, Bowman, Gary, Janies, Daniel A, Saif, Linda J
Format: Article
Language:English
Subjects:
Animal Husbandry
Animals
Biological and medical sciences
Caliciviridae Infections - veterinary
Caliciviridae Infections - virology
Capsid
Fundamental and applied biological sciences. Psychology
Gastroenteritis - veterinary
Gastroenteritis - virology
Genetic Variation
Humans
Infectious diseases
Medical sciences
Microbiology
Molecular Sequence Data
Phylogeny
Porcine sapovirus
Recombination, Genetic
RNA Replicase - genetics
Sapovirus - classification
Sapovirus - genetics
Sequence Analysis, DNA
Swine - virology
Swine Diseases - virology
Virology
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Summary:Sapoviruses (SaVs) are emerging enteric pathogens that cause diarrhea in humans and animals. Human SaVs are genetically variable and have been classified into four genogroups (GI, -II, -IV, and -V). To date, only two genetically similar porcine SaV strains have been reported that belong to GIII. To investigate the genetic diversity of porcine SaVs and their genetic relatedness to human strains, we sequenced 286 nucleotides (nt) of the RNA-dependent RNA polymerase (RdRp) region of nine porcine SaVs detected from field pig fecal samples collected in U.S. swine farms during the period from 1999 to 2003. One strain (Po/SaV/MI-QW19/2002/US) was most closely related to human GII SaVs. We also sequenced 3 kb of the viral genome, including the partial RdRp (766 to 790 nt), the complete capsid, the ORF2 and the 3'-untranslated region of four strains representative for the positive farms or for the distinct genetic clusters. From the sequence analysis of the complete capsid, we identified a potential new genogroup of porcine SaVs, with Po/SaV/OH-JJ681/00/US as the representative strain. Furthermore, two potential porcine SaV recombinants were identified. To our knowledge this is the first report of a porcine SaV strain more closely related genetically to human SaVs and the occurrence of porcine SaV recombinants. The presence of porcine SaVs more similar to human SaVs is a significant finding because of the potential for zoonotic infections or generation of porcine/human recombinants if intragenogroup human strains exist.
ISSN:0095-1137
1098-660X
1098-5530
DOI:10.1128/JCM.43.12.5963-5972.2005