Nocodazole inhibits macronuclear infection with Holospora obtusa in Paramecium caudatum

Holospora obtusa is a Gram-negative bacterium inhabiting the macronucleus of the ciliate Paramecium caudatum. Experimental infection with H. obtusa was carried out under nocodazole treatment. Nocodazole has been shown to cause disassembly of the cytoplasmic microtubules radiating from the cytopharyn...

Full description

Saved in:
Bibliographic Details
Published in:Protoplasma 2005-12, Vol.226 (3-4), p.147-153
Main Authors: Sabaneyeva, E V, Fokin, S I, Gavrilova, E V, Kornilova, E S
Format: Article
Language:English
Subjects:
Actin Cytoskeleton - drug effects
Actin Cytoskeleton - microbiology
Animals
Aquaculture
Cytoplasmic Streaming - drug effects
Holospora obtusa
Holosporaceae - drug effects
Holosporaceae - isolation & purification
Immunohistochemistry
In Situ Hybridization
Macronucleus - microbiology
Macronucleus - ultrastructure
Microtubules - drug effects
Microtubules - microbiology
Nocodazole - pharmacology
Paramecium caudatum
Paramecium caudatum - microbiology
Paramecium caudatum - ultrastructure
Phagocytosis - drug effects
Phagosomes - drug effects
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Holospora obtusa is a Gram-negative bacterium inhabiting the macronucleus of the ciliate Paramecium caudatum. Experimental infection with H. obtusa was carried out under nocodazole treatment. Nocodazole has been shown to cause disassembly of the cytoplasmic microtubules radiating from the cytopharynx and postoral fibers in P. caudatum. Treatment with this drug did not prevent the ingestion of both prey bacteria and H. obtusa, but it reduced the phagosome number and affected cyclosis. In situ hybridization revealed infectious forms of this endobiont very close to the macronucleus, but never inside it. These results indicate that disassembly of microtubules does not impair transportation of the infectious forms of H. obtusa in the cytoplasm, but that it completely blocks the invasion of the nucleus by the bacteria.
ISSN:0033-183X
1615-6102
DOI:10.1007/s00709-005-0121-7