Response of antioxidant defences to oxidative stress induced by prolonged exercise: antioxidant enzyme gene expression in lymphocytes

The response of lymphocyte and plasma antioxidant defences to a prolonged exercise as a cycling stage in a professional race was analysed. Antioxidant enzyme activities and gene expression, carbonyl derivative and MDA levels were determined in lymphocytes. Plasma levels of vitamin E, carotenes, prot...

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Bibliographic Details
Published in:European journal of applied physiology 2006-10, Vol.98 (3), p.263-269
Main Authors: Cases, Nuria, Sureda, Antoni, Maestre, Isabel, Tauler, Pedro, Aguiló, Antoni, Córdova, Alfredo, Roche, Enrique, Tur, Josep A, Pons, Antoni
Format: Article
Language:English
Subjects:
Antioxidants
Antioxidants - analysis
Biomarkers - analysis
Carotenoids - blood
Catalase - blood
Enzymes
Exercise
Glutathione Peroxidase - blood
Humans
Lymphocytes
Lymphocytes - enzymology
Male
Oxidative Stress
Serum - chemistry
Superoxide Dismutase - blood
Vitamin E
Vitamin E - blood
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Summary:The response of lymphocyte and plasma antioxidant defences to a prolonged exercise as a cycling stage in a professional race was analysed. Antioxidant enzyme activities and gene expression, carbonyl derivative and MDA levels were determined in lymphocytes. Plasma levels of vitamin E, carotenes, protein carbonyl derivatives and the test d-Roms were measured. Significant increases in plasmatic carbonyls and in the test d-Roms were observed after the cycling stage. No significant differences were found in the lymphocyte MDA and carbonyl derivative levels. A significant increase was found in plasma vitamin E concentration after the cycling stage; however, the lymphocyte vitamin E concentration did not change. Significant increases were observed in lymphocyte total superoxide dismutase (SOD) activity and in the levels of CuZn-SOD and Mn-SOD isoenzymes. The moderate levels of oxidative stress in the lymphocyte induced a cellular adaptation to exercise enough to counteract the negative effects of oxidative stress.
ISSN:1439-6319
1439-6327
DOI:10.1007/s00421-006-0273-y