Global Analysis of the Hortaea werneckii Proteome:  Studying Steroid Response in Yeast

The response of the halophilic black yeast Hortaea werneckii to the steroid hormone progesterone has been studied at the protein level using fluorescent two-dimensional differential gel electrophoresis (2D-DIGE) technology in combination with mass spectrometry. Data on protein identification from th...

Full description

Saved in:
Bibliographic Details
Published in:Journal of proteome research 2005-11, Vol.4 (6), p.2043-2051
Main Authors: Matis, Maja, Žakelj-Mavrič, Marija, Peter-Katalinić, Jasna
Format: Article
Language:English
Subjects:
Ascomycota - metabolism
DNA, Complementary - metabolism
Electrophoresis, Gel, Two-Dimensional
Fungal Proteins - chemistry
Gene Expression Regulation
Mass Spectrometry
Oligonucleotide Array Sequence Analysis
Phosphorylation
Progesterone - chemistry
Progesterone - metabolism
Proteins - chemistry
Proteome
Proteomics - methods
Saccharomyces cerevisiae - metabolism
Signal Transduction
Spectrometry, Fluorescence
Steroids - metabolism
Temperature
Time Factors
Up-Regulation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The response of the halophilic black yeast Hortaea werneckii to the steroid hormone progesterone has been studied at the protein level using fluorescent two-dimensional differential gel electrophoresis (2D-DIGE) technology in combination with mass spectrometry. Data on protein identification from this study reveal molecular mechanisms of the response to progesterone. In particular, the overexpression of Pck2 and Pac2 in the stimulated cells indicates the interactions of progesterone with the cell growth and reproduction signaling pathways. Keywords: fluorescent two-dimensional differential gel electrophoresis • de novo sequencing • mass spectrometry • phosphoproteome • steroids • stress response
ISSN:1535-3893
1535-3907
DOI:10.1021/pr050195f