Gene expression study in the juvenile mouse testis: identification of stage-specific molecular pathways during spermatogenesis

A gene expression time course in the juvenile mouse testis was established using cDNA microarrays derived from a variety of isolated testis cell types. In conjunction with the use of four germ cell-deficient mouse models, a stage and cell-type classification over nine time points has been obtained a...

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Bibliographic Details
Published in:Mammalian genome 2006-09, Vol.17 (9), p.956-975
Main Authors: Clemente, Emily J, Furlong, Robert A, Loveland, Kate L, Affara, Nabeel A
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Apoptosis - genetics
Cell Proliferation
Cells
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Developmental
Germ Cells - cytology
Germ Cells - metabolism
Male
Mice
Mice, Inbred C57BL
Rodents
Sertoli Cells - cytology
Sertoli Cells - metabolism
Signal Transduction - genetics
Signal Transduction - physiology
Spermatids - cytology
Spermatids - metabolism
Spermatocytes - cytology
Spermatocytes - metabolism
Spermatogenesis - genetics
Testis - cytology
Testis - growth & development
Testis - metabolism
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Summary:A gene expression time course in the juvenile mouse testis was established using cDNA microarrays derived from a variety of isolated testis cell types. In conjunction with the use of four germ cell-deficient mouse models, a stage and cell-type classification over nine time points has been obtained and analyzed for differential expression of genes. The expression profiles have been clustered into nine groups and subjected to detailed analysis of associated gene ontology. This has allowed the correlation of particular cellular processes and functions with different expression clusters. Focused analysis of transcripts involved in cell number regulation (apoptosis and proliferation) and their spatiotemporal expression patterns are presented. The findings indicate that for genes involved in both apoptosis and proliferation, several distinct pathways regulating these processes are active in somatic and germ cell lineages.
ISSN:0938-8990
1432-1777
DOI:10.1007/s00335-006-0029-3