Laser-induced fluorescence imaging and spectroscopy of GFP transgenic plants

Green fluorescent protein (GFP) and other fluorescent protein bioreporters can be used to monitor transgenes in plants. GFP is a valuable marker for transgene presence and expression, but remote sensing instrumentation for stand-off detection has lagged behind fluorescent protein marker biotechnolog...

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Bibliographic Details
Published in:Journal of fluorescence 2005-09, Vol.15 (5), p.697-705, Article 697
Main Authors: Stewart, Jr, C Neal, Millwood, Reginald J, Halfhill, Matthew D, Ayalew, Mentewab, Cardoza, Vinitha, Kooshki, Mitra, Capelle, Gene A, Kyle, Kevin R, Piaseki, David, McCrum, Gregory, Di Benedetto, John
Format: Article
Language:English
Subjects:
Green Fluorescent Proteins - analysis
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Lasers
Plants, Genetically Modified - chemistry
Plants, Genetically Modified - metabolism
Plants, Genetically Modified - radiation effects
Spectrometry, Fluorescence - methods
Transgenes
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Summary:Green fluorescent protein (GFP) and other fluorescent protein bioreporters can be used to monitor transgenes in plants. GFP is a valuable marker for transgene presence and expression, but remote sensing instrumentation for stand-off detection has lagged behind fluorescent protein marker biotechnology. However, both biology and photonics are needed for the monitoring technology to be fully realized. In this paper, we describe laser-induced fluorescence imaging and laser-induced fluorescence spectroscopy of GFP-transgenic plants in ambient light towards the application of remote sensing of transgenic plants producing GFP.
ISSN:1053-0509
1573-4994
DOI:10.1007/s10895-005-2977-5