Enantiomeric composition studies in Lavandula species using supercritical fluids

• Published in: Journal of separation science 2005-11, Vol.28 (17), p.2333-2338
• Main Authors: Flores, Gema, Blanch, Gracia Patricia, Ruiz del Castillo, Maria Luisa, Herraiz, Marta
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Applied sciences; Chemical industry and chemicals; Chemistry; Chiral; Chromatographic methods and physical methods associated with chromatography; Chromatography, Supercritical Fluid - methods; Enantiomer; Essential oils, perfumes; Exact sciences and technology; Gas chromatographic methods; Gas Chromatography-Mass Spectrometry - methods; Lavandula; Lavandula - chemistry; Lavandula - classification; Multidimensional gas chromatography; Oils, Volatile - analysis; Oils, Volatile - chemistry; Species Specificity; Stereoisomerism; Supercritical fluid extraction; Washing products. Cosmetics and toiletries. Perfumes
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.200500124

Characteristic aroma compounds in plants and essential oils of Lavandula from different varieties were examined. The study of the qualitative and quantitative composition of the major volatile components was faced by developing a method based on the use of supercritical fluid extraction‐GC‐MS (SFE‐GC‐MS). The optimization of a variety of parameters affecting SFE extraction enabled RSDs from three replicates lower than 2% to be achieved. Equally, recoveries of up to 59% were obtained by applying the proposed method. The use of multidimensional GC was necessary to enantiomerically resolve the target compounds. The obtained results showed enantiomeric purities >90% for all studied compounds in all varieties considered, proving the natural invariability of the enantiomeric composition of the compounds of interest. This information can be useful in authenticity studies as well as in selecting natural sources of enantiomerically pure compounds.