Diet-related variation in cellular retinol-binding protein type II gene expression in rat jejunum

• Published in: British journal of nutrition 2005-12, Vol.94 (6), p.890-895
• Main Authors: Suruga, Kazuhito, Kitagawa, Masaaki, Yasutake, Hiromitsu, Takase, Sachiko, Goda, Toshinao
• Format: Article
• Language: English
• Subjects: animal models; Animals; Biological and medical sciences; Carbohydrates; cell physiology; Circadian Rhythm - physiology; CRBPII; Diet; dietary fat; Dietary Fats - administration & dosage; Eating - physiology; fatty acid-binding proteins; Fatty Acid-Binding Proteins - analysis; Fatty acids; Fatty Acids - metabolism; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; Gene Expression; high fat diet; intestinal absorption; jejunum; Jejunum - cytology; Jejunum - metabolism; L-FABP; Light; lipolysis; liver fatty acid-binding protein; low fat diet; Male; messenger RNA; Metabolism; Nutrition research; nutrition-genotype interaction; Oils & fats; PPAR; PPAR alpha - metabolism; promoter regions; Proteins; Rats; Rats, Wistar; Retinoid X Receptor alpha - metabolism; retinol-binding protein; Retinol-Binding Proteins - genetics; Retinol-Binding Proteins - metabolism; Retinol-Binding Proteins, Cellular; Small intestine; transcriptional activation; Transcriptional Activation - genetics; ultradian rhythm; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Vitamin A; vitamin metabolism
• ISSN: 0007-1145; 1475-2662
• DOI: 10.1079/BJN20051575

Cellular retinol-binding protein type II (CRBPII) is involved in the transport of vitamin A and its metabolism in the small intestine. In the present study, we demonstrated diet-related variations in CRBPII expression in rat jejunum. The CRBPII protein and mRNA levels increased in parallel after the start of feeding period regardless of whether the feeding period was restricted to the hours of darkness or of light. In addition, this variation was observed in the rats fed high-fat diet or low-fat diets, but not in those fed a fat-free diet or in fasted rats. A similar diet-induced variation was seen in the mRNA of liver-type fatty acid-binding protein in rat jejunum. In the transient transfection experiment, unsaturated fatty acid increased rat CRBPII gene promoter activity via the PPARα/retinoid X receptor-α heterodimer. Taken together, these results suggest that the diet-related variation in CRBPII expression in rat jejunum may be brought about by the transcriptional induction of CRBPII gene expression mainly triggered by dietary fatty acids.