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Class 1 integrons in Dutch Salmonella enterica serovar Dublin isolates from clinical cases of bovine salmonellosis
Fifty-nine Salmonella enterica serovar Dublin ( Salmonella Dublin) isolates from clinical cases of bovine salmonellosis between 1993 and 2004 were tested for their susceptibility to 15 antimicrobial agents and the presence of class 1 integrons. Integrons were further analyzed by conserved segment PC...
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| Published in: | Veterinary microbiology 2006-10, Vol.117 (2), p.192-200 |
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| container_title | Veterinary microbiology |
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| creator | Vo, An T.T. van Duijkeren, Engeline Fluit, Ad C. Heck, Max E.O.C. Verbruggen, Anjo van der Zwaluw, Kim Gaastra, Wim |
| description | Fifty-nine
Salmonella enterica serovar Dublin (
Salmonella Dublin) isolates from clinical cases of bovine salmonellosis between 1993 and 2004 were tested for their susceptibility to 15 antimicrobial agents and the presence of class 1 integrons. Integrons were further analyzed by conserved segment PCR-RFLP. DNA sequencing was used to identify the inserted gene cassette. Twelve (20.3%) isolates were multidrug-resistant. A combination of resistance against chloramphenicol, streptomycin and sulphonamides was the most common phenotype observed. Multidrug-resistance (MDR) was found to be strongly associated with the presence of integrons, since a class 1 integron with the
aadA1 gene cassette encoding resistance to streptomycin and spectinomycin was found in all 12 multidrug-resistant isolates. The presence of the
aadA1 gene in
Salmonella Dublin has not been reported before. None of the integron carrying
Salmonella Dublin isolates could transfer its antimicrobial resistance to
E. coli K12 by conjugation. Analysis of plasmid profiles and pulsed field gel electrophoresis (PFGE) patterns showed at least some clonality among the
Salmonella Dublin isolates, but 11 different types could be distinguished based on both
XbaI and
BlnI-PFGE patterns. Thus, the Dutch
Salmonella Dublin strains were closely related but not clonal. |
| doi_str_mv | 10.1016/j.vetmic.2006.05.001 |
| format | article |
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Salmonella enterica serovar Dublin (
Salmonella Dublin) isolates from clinical cases of bovine salmonellosis between 1993 and 2004 were tested for their susceptibility to 15 antimicrobial agents and the presence of class 1 integrons. Integrons were further analyzed by conserved segment PCR-RFLP. DNA sequencing was used to identify the inserted gene cassette. Twelve (20.3%) isolates were multidrug-resistant. A combination of resistance against chloramphenicol, streptomycin and sulphonamides was the most common phenotype observed. Multidrug-resistance (MDR) was found to be strongly associated with the presence of integrons, since a class 1 integron with the
aadA1 gene cassette encoding resistance to streptomycin and spectinomycin was found in all 12 multidrug-resistant isolates. The presence of the
aadA1 gene in
Salmonella Dublin has not been reported before. None of the integron carrying
Salmonella Dublin isolates could transfer its antimicrobial resistance to
E. coli K12 by conjugation. Analysis of plasmid profiles and pulsed field gel electrophoresis (PFGE) patterns showed at least some clonality among the
Salmonella Dublin isolates, but 11 different types could be distinguished based on both
XbaI and
BlnI-PFGE patterns. Thus, the Dutch
Salmonella Dublin strains were closely related but not clonal.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2006.05.001</identifier><identifier>PMID: 16781090</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; antibiotic resistance ; antibiotics ; Antimicrobial resistance ; Bacterial diseases ; Bacterial diseases of the digestive system and abdomen ; Bacteriology ; Biological and medical sciences ; Cattle ; cattle diseases ; Cattle Diseases - drug therapy ; Cattle Diseases - epidemiology ; Conjugation, Genetic ; Drug Resistance, Bacterial - genetics ; Drug Resistance, Multiple, Bacterial - genetics ; Electrophoresis, Gel, Pulsed-Field - methods ; Electrophoresis, Gel, Pulsed-Field - veterinary ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; gene cassettes ; Genotype ; Genotyping ; Human bacterial diseases ; Infectious diseases ; Integron ; Integrons ; Medical sciences ; microbial genetics ; Microbial Sensitivity Tests - veterinary ; Microbiology ; Miscellaneous ; molecular sequence data ; multiple drug resistance ; nucleotide sequences ; plasmids ; Polymerase Chain Reaction - methods ; Polymerase Chain Reaction - veterinary ; Polymorphism, Restriction Fragment Length ; restriction fragment length polymorphism ; Salmonella ; Salmonella Dublin ; Salmonella enterica ; Salmonella enterica - drug effects ; Salmonella enterica - genetics ; Salmonella enterica subsp. enterica serovar Dublin ; Salmonella Infections, Animal - drug therapy ; Salmonella Infections, Animal - microbiology ; salmonellosis ; serotypes ; Treatment Outcome</subject><ispartof>Veterinary microbiology, 2006-10, Vol.117 (2), p.192-200</ispartof><rights>2006 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-93322e5111099343f231d851be347c0df543eb1e3aaba9221ff788ff08102d8c3</citedby><cites>FETCH-LOGICAL-c445t-93322e5111099343f231d851be347c0df543eb1e3aaba9221ff788ff08102d8c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18185405$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16781090$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vo, An T.T.</creatorcontrib><creatorcontrib>van Duijkeren, Engeline</creatorcontrib><creatorcontrib>Fluit, Ad C.</creatorcontrib><creatorcontrib>Heck, Max E.O.C.</creatorcontrib><creatorcontrib>Verbruggen, Anjo</creatorcontrib><creatorcontrib>van der Zwaluw, Kim</creatorcontrib><creatorcontrib>Gaastra, Wim</creatorcontrib><title>Class 1 integrons in Dutch Salmonella enterica serovar Dublin isolates from clinical cases of bovine salmonellosis</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>Fifty-nine
Salmonella enterica serovar Dublin (
Salmonella Dublin) isolates from clinical cases of bovine salmonellosis between 1993 and 2004 were tested for their susceptibility to 15 antimicrobial agents and the presence of class 1 integrons. Integrons were further analyzed by conserved segment PCR-RFLP. DNA sequencing was used to identify the inserted gene cassette. Twelve (20.3%) isolates were multidrug-resistant. A combination of resistance against chloramphenicol, streptomycin and sulphonamides was the most common phenotype observed. Multidrug-resistance (MDR) was found to be strongly associated with the presence of integrons, since a class 1 integron with the
aadA1 gene cassette encoding resistance to streptomycin and spectinomycin was found in all 12 multidrug-resistant isolates. The presence of the
aadA1 gene in
Salmonella Dublin has not been reported before. None of the integron carrying
Salmonella Dublin isolates could transfer its antimicrobial resistance to
E. coli K12 by conjugation. Analysis of plasmid profiles and pulsed field gel electrophoresis (PFGE) patterns showed at least some clonality among the
Salmonella Dublin isolates, but 11 different types could be distinguished based on both
XbaI and
BlnI-PFGE patterns. Thus, the Dutch
Salmonella Dublin strains were closely related but not clonal.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>antibiotic resistance</subject><subject>antibiotics</subject><subject>Antimicrobial resistance</subject><subject>Bacterial diseases</subject><subject>Bacterial diseases of the digestive system and abdomen</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>cattle diseases</subject><subject>Cattle Diseases - drug therapy</subject><subject>Cattle Diseases - epidemiology</subject><subject>Conjugation, Genetic</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Drug Resistance, Multiple, Bacterial - genetics</subject><subject>Electrophoresis, Gel, Pulsed-Field - methods</subject><subject>Electrophoresis, Gel, Pulsed-Field - veterinary</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene cassettes</subject><subject>Genotype</subject><subject>Genotyping</subject><subject>Human bacterial diseases</subject><subject>Infectious diseases</subject><subject>Integron</subject><subject>Integrons</subject><subject>Medical sciences</subject><subject>microbial genetics</subject><subject>Microbial Sensitivity Tests - veterinary</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>molecular sequence data</subject><subject>multiple drug resistance</subject><subject>nucleotide sequences</subject><subject>plasmids</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>restriction fragment length polymorphism</subject><subject>Salmonella</subject><subject>Salmonella Dublin</subject><subject>Salmonella enterica</subject><subject>Salmonella enterica - drug effects</subject><subject>Salmonella enterica - genetics</subject><subject>Salmonella enterica subsp. enterica serovar Dublin</subject><subject>Salmonella Infections, Animal - drug therapy</subject><subject>Salmonella Infections, Animal - microbiology</subject><subject>salmonellosis</subject><subject>serotypes</subject><subject>Treatment Outcome</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFkU2P0zAQhiMEYsvCP0DgC9waxh9JnQvSqnxKK3FY9mw5znhxlcSLJ63Ev2dKi_YGJ1szz7zz8VbVSwm1BNm-29UHXKYUagXQ1tDUAPJRtZJ2o9eqMepxtQK9sWspdXNRPSPaAYDpWnhaXch2YyV0sKrKdvREQoo0L3hX8kz8Ex_2S_ghbvw45RnH0QvkbEnBC8KSD74w0Y8MJsqjX5BELHkSgUMMjSJ44liOos-HNKOgv0qZEj2vnkQ_Er44v5fV7aeP37df1tffPn_dXl2vgzHNsu60VgobKXnQThsdlZaDbWSP2mwCDLExGnuJ2vved0rJGDfWxgi8mRps0JfV25Pufck_90iLmxKF4zoz5j251trOsNZ_QQWK-5qWQXMCQ8lEBaO7L2ny5ZeT4I6muJ07meKOpjhoHJvCZa_O-vt-wuGh6OwCA2_OgCc-Xyx-DokeOCttY6Bh7vWJiz47f1eYub1R3AH4StL-afX-RCAf9pCwOAoJ54BDKhgWN-T071l_A0nStgM</recordid><startdate>20061031</startdate><enddate>20061031</enddate><creator>Vo, An T.T.</creator><creator>van Duijkeren, Engeline</creator><creator>Fluit, Ad C.</creator><creator>Heck, Max E.O.C.</creator><creator>Verbruggen, Anjo</creator><creator>van der Zwaluw, Kim</creator><creator>Gaastra, Wim</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20061031</creationdate><title>Class 1 integrons in Dutch Salmonella enterica serovar Dublin isolates from clinical cases of bovine salmonellosis</title><author>Vo, An T.T. ; van Duijkeren, Engeline ; Fluit, Ad C. ; Heck, Max E.O.C. ; Verbruggen, Anjo ; van der Zwaluw, Kim ; Gaastra, Wim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-93322e5111099343f231d851be347c0df543eb1e3aaba9221ff788ff08102d8c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>antibiotic resistance</topic><topic>antibiotics</topic><topic>Antimicrobial resistance</topic><topic>Bacterial diseases</topic><topic>Bacterial diseases of the digestive system and abdomen</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>cattle diseases</topic><topic>Cattle Diseases - drug therapy</topic><topic>Cattle Diseases - epidemiology</topic><topic>Conjugation, Genetic</topic><topic>Drug Resistance, Bacterial - genetics</topic><topic>Drug Resistance, Multiple, Bacterial - genetics</topic><topic>Electrophoresis, Gel, Pulsed-Field - methods</topic><topic>Electrophoresis, Gel, Pulsed-Field - veterinary</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene cassettes</topic><topic>Genotype</topic><topic>Genotyping</topic><topic>Human bacterial diseases</topic><topic>Infectious diseases</topic><topic>Integron</topic><topic>Integrons</topic><topic>Medical sciences</topic><topic>microbial genetics</topic><topic>Microbial Sensitivity Tests - veterinary</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>molecular sequence data</topic><topic>multiple drug resistance</topic><topic>nucleotide sequences</topic><topic>plasmids</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>restriction fragment length polymorphism</topic><topic>Salmonella</topic><topic>Salmonella Dublin</topic><topic>Salmonella enterica</topic><topic>Salmonella enterica - drug effects</topic><topic>Salmonella enterica - genetics</topic><topic>Salmonella enterica subsp. enterica serovar Dublin</topic><topic>Salmonella Infections, Animal - drug therapy</topic><topic>Salmonella Infections, Animal - microbiology</topic><topic>salmonellosis</topic><topic>serotypes</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vo, An T.T.</creatorcontrib><creatorcontrib>van Duijkeren, Engeline</creatorcontrib><creatorcontrib>Fluit, Ad C.</creatorcontrib><creatorcontrib>Heck, Max E.O.C.</creatorcontrib><creatorcontrib>Verbruggen, Anjo</creatorcontrib><creatorcontrib>van der Zwaluw, Kim</creatorcontrib><creatorcontrib>Gaastra, Wim</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vo, An T.T.</au><au>van Duijkeren, Engeline</au><au>Fluit, Ad C.</au><au>Heck, Max E.O.C.</au><au>Verbruggen, Anjo</au><au>van der Zwaluw, Kim</au><au>Gaastra, Wim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Class 1 integrons in Dutch Salmonella enterica serovar Dublin isolates from clinical cases of bovine salmonellosis</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2006-10-31</date><risdate>2006</risdate><volume>117</volume><issue>2</issue><spage>192</spage><epage>200</epage><pages>192-200</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>Fifty-nine
Salmonella enterica serovar Dublin (
Salmonella Dublin) isolates from clinical cases of bovine salmonellosis between 1993 and 2004 were tested for their susceptibility to 15 antimicrobial agents and the presence of class 1 integrons. Integrons were further analyzed by conserved segment PCR-RFLP. DNA sequencing was used to identify the inserted gene cassette. Twelve (20.3%) isolates were multidrug-resistant. A combination of resistance against chloramphenicol, streptomycin and sulphonamides was the most common phenotype observed. Multidrug-resistance (MDR) was found to be strongly associated with the presence of integrons, since a class 1 integron with the
aadA1 gene cassette encoding resistance to streptomycin and spectinomycin was found in all 12 multidrug-resistant isolates. The presence of the
aadA1 gene in
Salmonella Dublin has not been reported before. None of the integron carrying
Salmonella Dublin isolates could transfer its antimicrobial resistance to
E. coli K12 by conjugation. Analysis of plasmid profiles and pulsed field gel electrophoresis (PFGE) patterns showed at least some clonality among the
Salmonella Dublin isolates, but 11 different types could be distinguished based on both
XbaI and
BlnI-PFGE patterns. Thus, the Dutch
Salmonella Dublin strains were closely related but not clonal.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16781090</pmid><doi>10.1016/j.vetmic.2006.05.001</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1135 |
| ispartof | Veterinary microbiology, 2006-10, Vol.117 (2), p.192-200 |
| issn | 0378-1135 1873-2542 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68894347 |
| source | Elsevier |
| subjects | Animals Anti-Bacterial Agents - pharmacology antibiotic resistance antibiotics Antimicrobial resistance Bacterial diseases Bacterial diseases of the digestive system and abdomen Bacteriology Biological and medical sciences Cattle cattle diseases Cattle Diseases - drug therapy Cattle Diseases - epidemiology Conjugation, Genetic Drug Resistance, Bacterial - genetics Drug Resistance, Multiple, Bacterial - genetics Electrophoresis, Gel, Pulsed-Field - methods Electrophoresis, Gel, Pulsed-Field - veterinary Escherichia coli Fundamental and applied biological sciences. Psychology gene cassettes Genotype Genotyping Human bacterial diseases Infectious diseases Integron Integrons Medical sciences microbial genetics Microbial Sensitivity Tests - veterinary Microbiology Miscellaneous molecular sequence data multiple drug resistance nucleotide sequences plasmids Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Polymorphism, Restriction Fragment Length restriction fragment length polymorphism Salmonella Salmonella Dublin Salmonella enterica Salmonella enterica - drug effects Salmonella enterica - genetics Salmonella enterica subsp. enterica serovar Dublin Salmonella Infections, Animal - drug therapy Salmonella Infections, Animal - microbiology salmonellosis serotypes Treatment Outcome |
| title | Class 1 integrons in Dutch Salmonella enterica serovar Dublin isolates from clinical cases of bovine salmonellosis |
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