Chemically Selective Sensing through Layer-by-Layer Incorporation of Biorecognition into Thin Film Substrates for Surface-Enhanced Resonance Raman Scattering

In this work, the fabrication, characterization, and application of avidin/Ag nanoparticle layer-by-layer (LbL) films as chemically selective substrates for surface-enhanced resonance Raman scattering (SERRS) is demonstrated. The biospecific interaction between avidin and the small molecule biotin,...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2006-10, Vol.128 (39), p.12626-12627
Main Authors: Pieczonka, Nicholas P. W, Goulet, Paul J. G, Aroca, Ricardo F
Format: Article
Language:English
Subjects:
Avidin - chemistry
Biotin - chemistry
Chemistry
Colloids - chemistry
Exact sciences and technology
General and physical chemistry
Microscopy, Atomic Force
Models, Molecular
Nanoparticles - chemistry
Silver - chemistry
Spectrum Analysis, Raman - methods
Surface Plasmon Resonance - methods
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Summary:In this work, the fabrication, characterization, and application of avidin/Ag nanoparticle layer-by-layer (LbL) films as chemically selective substrates for surface-enhanced resonance Raman scattering (SERRS) is demonstrated. The biospecific interaction between avidin and the small molecule biotin, one of the strongest known to exist in nature, is exploited to preferentially capture biotinylated species from solution. This highly favored adsorption is shown to yield SERRS concentration enhancements and improved detection sensitivities of ca. 102 for commercially available and in situ prepared biotinylated species over their nontagged counterparts.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja0644327