Low-Cost Fermentation Medium for Thermophilic Protease Production by Streptomyces sp. 594 Using Feather Meal and Corn Steep Liquor

• Published in: Current microbiology 2006-10, Vol.53 (4), p.335-339
• Main Authors: Azeredo, L. A. I. De, Lima, M. B. De, Coelho, R. R. R, Freire, D. M. G
• Format: Article
• Language: English
• Subjects: Animals; Bacteriology; Corn; Culture Media; Feathers; Fermentation; High temperature; Hydrogen-Ion Concentration; Industrial Microbiology - economics; Industrial Microbiology - methods; Microbiology; Peptide Hydrolases - biosynthesis; Proteases; Streptomyces; Streptomyces - enzymology; Streptomyces - isolation & purification; Streptomyces - metabolism; Streptomycetes; Temperature; Yeast; Yeasts; Zea mays - chemistry
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-006-0163-x

Protease production by Streptomyces sp. 594 was obtained after submerged fermentation (SF) and solid-state fermentation (SSF) using feather meal (FM) and corn steep liquor (CSL) as sole sources of carbon and nitrogen. Enzyme productions were 13.4 U ml-¹ in SF and 21.5 U g-¹ in SSF; these values were approximately 86% and 39% higher, respectively, than those obtained previously when yeast extract was used in place of CSL. The proteases, which belong to the serine and metalloproteinase classes, were active at high temperatures (55°C to 90°C) and over a wide range of pH values (5.0 to 10.0). Thus, these thermophilic proteases have shown interesting properties for industrial purposes. As far as we are concerned, this is the first contribution toward the microbial production of thermophilic proteases by a streptomycete using a low-cost medium composed of industrial poultry (FM) and corn processing by-products (CSL).