Behavior of Polycatalytic Assemblies in a Substrate-Displaying Matrix

We describe polycatalytic assemblies, comprising one or two streptavidin molecules and two to six attached nucleic acid catalysts (deoxyribozymes), with phosphodiesterase activity. When exposed to a matrix covered at high densities with oligonucleotide substrates, these molecules diffuse through the...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2006-10, Vol.128 (39), p.12693-12699
Main Authors: Pei, Renjun, Taylor, Steven K, Stefanovic, Darko, Rudchenko, Sergei, Mitchell, Tiffany E, Stojanovic, Milan N
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carbohydrates. Nucleosides and nucleotides
Catalysis
Catalysts: preparations and properties
Chemistry
Dextrans - chemistry
DNA, Catalytic - chemistry
DNA, Catalytic - metabolism
Dna, deoxyribonucleoproteins
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General and physical chemistry
Hydrogels - chemistry
Nucleic acids
Nucleosides, nucleotides and oligonucleotides
Oligonucleotides - chemistry
Organic chemistry
Phosphoric Diester Hydrolases - chemistry
Phosphoric Diester Hydrolases - metabolism
Preparations and properties
Streptavidin - chemistry
Surface Plasmon Resonance
Theory of reactions, general kinetics. Catalysis. Nomenclature, chemical documentation, computer chemistry
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Summary:We describe polycatalytic assemblies, comprising one or two streptavidin molecules and two to six attached nucleic acid catalysts (deoxyribozymes), with phosphodiesterase activity. When exposed to a matrix covered at high densities with oligonucleotide substrates, these molecules diffuse through the matrix continuously cleaving the substrate at rates comparable to those of individual catalysts in solution. Rates of diffusion (movement), processivity, and resident times of assemblies can be controlled through the number of catalytic units and the length of substrate/product recognition regions. The assemblies were characterized at the ensemble level using surface plasmon resonance.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja058394n