High-Throughput Relative DPPH Radical Scavenging Capacity Assay

• Published in: Journal of agricultural and food chemistry 2006-10, Vol.54 (20), p.7429-7436
• Main Authors: Cheng, Zhihong, Moore, Jeffrey, Yu, Liangli (Lucy)
• Format: Article
• Language: English
• Subjects: antioxidant activity; antioxidants; Antioxidants - analysis; assays; Biological and medical sciences; Biphenyl Compounds; Colorimetry; DPPH radical scavenging; flour; food chemistry; Food industries; Free Radical Scavengers - chemistry; free radicals; Fundamental and applied biological sciences. Psychology; grapes; Kinetics; Picrates - chemistry; raspberries; red wheat; Reproducibility of Results; seeds; Seeds - chemistry; Sensitivity and Specificity; Spectrophotometry; Triticum - chemistry; Vitis - chemistry; winter wheat
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf0611668

A high-throughput relative 2,2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging capacity (RDSC) assay was developed and validated in the present study. This RDSC assay is easy to perform and has acceptable accuracy (90−110% recovery), precision [3.9−7.0% pooled relative standard deviation (RSD)], and reproducibility (2.2 and 3.5% interday and intraday RSD). This assay reports the RDSC values for antioxidant samples, which make it possible to compare the DPPH radical scavenging capacities of antioxidants determined in different laboratories. The RDSC assay may be conducted in aqueous alcohol and acetone for hydrophilic antioxidants or in the organic solvents for lipophilic antioxidants without solubilizing agents, which makes it possible to directly compare the radical scavenging capacities of hydrophilic and lipophilic antioxidants. In addition, the high-throughput RDSC assay could be utilized for EC50 value estimation. The high-throughput RDSC assay may be used for screening and investigating potential natural antioxidants. Keywords: Radical scavenging capacity; RDSC, DPPH radical; antioxidant activity; high-throughput; AUC