Rapid solid-phase peptide synthesis using thermal and controlled microwave irradiation

A rapid and efficient microwave‐assisted solid‐phase synthesis method is described for the preparation of the nonapeptide WDTVRISFK, using conventional Fmoc/But orthogonal protection strategy. The synthesis protocol is based on the use of cycles of pulsed microwave irradiation with intermittent cool...

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Published in:Journal of peptide science 2006-10, Vol.12 (10), p.633-638
Main Authors: Bacsa, Bernadett, Desai, Bimbisar, Dibó, GáBor, Kappe, C. Oliver
Format: Article
Language:English
Subjects:
Biochemistry - methods
MicroKan
microwave irradiation
microwave-assisted organic synthesis
microwave-assisted peptide synthesis
Microwaves
Peptides - chemical synthesis
Peptides - chemistry
solid-phase peptide synthesis
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container_title Journal of peptide science
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creator Bacsa, Bernadett
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Dibó, GáBor
Kappe, C. Oliver
description A rapid and efficient microwave‐assisted solid‐phase synthesis method is described for the preparation of the nonapeptide WDTVRISFK, using conventional Fmoc/But orthogonal protection strategy. The synthesis protocol is based on the use of cycles of pulsed microwave irradiation with intermittent cooling of the reaction during the removal of the Fmoc protecting group and during the coupling. The desired nonapeptide was obtained in highest yield and purity by employing MicroKan technology. The chemical reactions were carried out in a single‐mode microwave reactor, equipped with a fiber‐optic probe to monitor the reaction temperature continuously. Copyright © 2006 European Peptide Society and John Wiley & Sons, Ltd.
doi_str_mv 10.1002/psc.771
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source Wiley-Blackwell Read & Publish Collection
subjects Biochemistry - methods
MicroKan
microwave irradiation
microwave-assisted organic synthesis
microwave-assisted peptide synthesis
Microwaves
Peptides - chemical synthesis
Peptides - chemistry
solid-phase peptide synthesis
title Rapid solid-phase peptide synthesis using thermal and controlled microwave irradiation
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