Temperature-induced cell detachment on immobilized pluronic surface

The Pluronic F68 and F127, a triblock copolymer of ethylene oxide and propylene oxide, was activated using carbonyldiimidazole (CDI), and CDI‐activated Pluronic F68 and F127 was subsequently immobilized on the surface of a poly‐L‐lysine‐coated polystyrene tissue culture flask. Cell culture was perfo...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomedical materials research 2006-11, Vol.79A (2), p.380-392
Main Authors: Higuchi, Akon, Aoki, Nobuo, Yamamoto, Taro, Miyazaki, Toyohiko, Fukushima, Hisashi, Tak, Tae Moon, Jyujyoji, Shin, Egashira, Satsuki, Matsuoka, Yuki, Natori, Shizue Hayashi
Format: Article
Language:English
Subjects:
AC133 Antigen
Animals
Antigens, CD - biosynthesis
Antigens, CD34 - biosynthesis
Biocompatible Materials
cell adhesion
cell culture
Fetal Blood - metabolism
Glycoproteins - biosynthesis
Hematopoietic Stem Cells - cytology
hydrophilicity
Imidazoles - chemistry
Mice
Peptides
Poloxamer - chemistry
Polylysine - chemistry
Polystyrenes - chemistry
stem cell
surface modification
Surface Properties
Temperature
Umbilical Veins - cytology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The Pluronic F68 and F127, a triblock copolymer of ethylene oxide and propylene oxide, was activated using carbonyldiimidazole (CDI), and CDI‐activated Pluronic F68 and F127 was subsequently immobilized on the surface of a poly‐L‐lysine‐coated polystyrene tissue culture flask. Cell culture was performed on the Pluronic‐immobilized flask. The morphology of fibroblasts (L929 cells) on the Pluronic F127‐immobilized flask was mainly spherical, and showed less spreading behavior than that on the Pluronic F68‐immobilized flask and conventional tissue culture flask. This observation indicates that L929 cells on Pluronic F127‐immobilized flasks were cultured in a bio‐inert environment. L929 cells were successively detached from both Pluronic F127‐immobilized flask and Pluronic F68‐immobilized flask by cooling the flask to 4–15°C. This detachment is due to the hydration and dehydration properties of Pluronic, depending on the temperature. Umbilical cord blood was cultured in the Pluronic F127‐immobilized and conventional polystyrene tissue culture flasks at 37°C. The expression ratio of surface markers on hematopoietic stem cells (CD34 and CD133) cultured in the Pluronic F127‐immobilized flask was significantly higher than that of the cells in polystyrene tissue culture flask. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006
ISSN:1549-3296
0021-9304
1552-4965
1097-4636
DOI:10.1002/jbm.a.30773