Global analysis of gene expression in Xenopus hindlimbs during stage‐dependent complete and incomplete regeneration

Xenopus laevis tadpoles are capable of limb regeneration after amputation, in a process that initially involves the formation of a blastema. However, Xenopus has full regenerative capacity only through premetamorphic stages. We have used the Affymetrix Xenopus laevis Genome Genechip microarray to pe...

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Bibliographic Details
Published in:Developmental dynamics 2006-10, Vol.235 (10), p.2667-2685
Main Authors: Grow, Matthew, Neff, Anton W., Mescher, Anthony L., King, Michael W.
Format: Article
Language:English
Subjects:
Affymetrix Genechip
Animals
blastema
gene expression
Gene Expression - genetics
Gene Expression Profiling - methods
Hindlimb - metabolism
Hindlimb - physiology
limb
Models, Theoretical
Oligonucleotide Array Sequence Analysis - methods
profiling
regeneration
Regeneration - genetics
Regeneration - physiology
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction - methods
Xenopus laevis
Xenopus laevis - genetics
Xenopus laevis - physiology
Xenopus Proteins - genetics
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Summary:Xenopus laevis tadpoles are capable of limb regeneration after amputation, in a process that initially involves the formation of a blastema. However, Xenopus has full regenerative capacity only through premetamorphic stages. We have used the Affymetrix Xenopus laevis Genome Genechip microarray to perform a large‐scale screen of gene expression in the regeneration‐complete, stage 53 (st53), and regeneration‐incomplete, stage 57 (st57), hindlimbs at 1 and 5 days postamputation. Through an exhaustive reannotation of the Genechip and a variety of comparative bioinformatic analyses, we have identified genes that are differentially expressed between the regeneration‐complete and ‐incomplete stages, detected the transcriptional changes associated with the regenerating blastema, and compared these results with those of other regeneration researchers. We focus particular attention on striking transcriptional activity observed in genes associated with patterning, stress response, and inflammation. Overall, this work provides the most comprehensive views yet of a regenerating limb and different transcriptional compositions of regeneration‐competent and deficient tissues. Developmental Dynamics 235:2667–2685, 2006. © 2006 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.20897