Legionella pneumophila Serogroup 1 Isolates from Cooling Towers in Japan Form a Distinct Genetic Cluster

Thirty‐one epidemiologically unrelated Legionella pneumophila serogroup 1 isolates (10 from cooling towers, 10 from public spas and/or hot spring baths, and 11 from patients) were analyzed by pulsed‐field gel electrophoresis (PFGE) and sequence‐based typing (SBT) using 6 loci, flaA, pilE, asd, mip,...

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Bibliographic Details
Published in:Microbiology and immunology 2005-01, Vol.49 (12), p.1027-1033
Main Authors: Amemura-Maekawa, Junko, Kura, Fumiaki, Chang, Bin, Watanabe, Haruo
Format: Article
Language:English
Subjects:
Adult
Aged
Bacterial Typing Techniques
DNA Primers
DNA, Bacterial - genetics
Electrophoresis, Gel, Pulsed-Field - methods
Environmental Microbiology
Female
Genes, Bacterial
Humans
Japan
Legionella pneumophila
Legionella pneumophila - classification
Legionella pneumophila - isolation & purification
Legionnaires' Disease - diagnosis
Legionnaires' Disease - microbiology
Legionnaires' Disease - transmission
Male
Middle Aged
Molecular Sequence Data
molecular typing
pulsed-field gel electrophoresis (PFGE)
Recreation
Sequence Analysis, DNA - methods
sequence-based typing (SBT)
Serotyping
Water Supply
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Summary:Thirty‐one epidemiologically unrelated Legionella pneumophila serogroup 1 isolates (10 from cooling towers, 10 from public spas and/or hot spring baths, and 11 from patients) were analyzed by pulsed‐field gel electrophoresis (PFGE) and sequence‐based typing (SBT) using 6 loci, flaA, pilE, asd, mip, mompS, and proA. The results of PFGE and SBT analysis indicated that all 10 isolates from cooling towers clustered into a unique type, which was distinct from strains of other environmental sources.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.2005.tb03699.x