Pyruvate kinase activation and lipoperoxidation after selective hepatic ischemia in Wistar rats

Hepatic ischemia and reperfusion can cause several problems in hepatic surgery. The aim of this study was to determine pyruvate kinase activation and lipid peroxidation after hepatic ischemia. Twenty-four Wistar rats were submitted to 90 minutes of selective liver ischemia and 15 minutes of reperfus...

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Published in:Acta cirúrgica brasileira 2006, Vol.21 Suppl 1 (suppl 1), p.19-23
Main Authors: Boin, Ilka de Faátima Santana Ferreira, Silva, Orlando de Castro e, Souza, Maria Eliza Jordani de, Santos, Antonio Cardoso, Leonardi, Luiz Sergio
Format: Article
Language:English
Subjects:
Animals
Blood Glucose - metabolism
Energy Metabolism - physiology
Enzyme Activation
Ischemia - complications
Ischemia - metabolism
Lipid Peroxidation - physiology
Liver - blood supply
Liver Circulation - drug effects
Liver Circulation - physiology
Liver Glycogen - metabolism
Male
Mitochondria, Liver - enzymology
Pyruvate Kinase - metabolism
Rats
Rats, Wistar
Reperfusion Injury - etiology
Reperfusion Injury - metabolism
Syndrome
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Summary:Hepatic ischemia and reperfusion can cause several problems in hepatic surgery. The aim of this study was to determine pyruvate kinase activation and lipid peroxidation after hepatic ischemia. Twenty-four Wistar rats were submitted to 90 minutes of selective liver ischemia and 15 minutes of reperfusion. Twelve animals were submitted to selective liver ischemia and reperfusion (Group A) and the other 12 were submitted to sham operation (Group B). After 15 minutes of reperfusion, the following parameters were measured: mean arterial pressure (MAP), alanine aminotransferase (ALT), glycemia (GLY), hepatic glycogen (GH), pyruvate kinase (PK) activation, hepatic glutathione (GSH) and malondialdehyde (MDA). Analysis of the results were made by the Student t-test and has been considered significant difference for p
ISSN:0102-8650
0102-8650
DOI:10.1590/S0102-86502006000700005