Crystallochemistry, textural properties, and in vitro biocompatibility of different silicon-doped calcium phosphates

• Published in: Journal of biomedical materials research 2006-09, Vol.78A (4), p.762-771
• Main Authors: Arcos, D., Sánchez-Salcedo, S., Izquierdo-Barba, I., Ruiz, L., González-Calbet, J., Vallet-Regí, M.
• Format: Article
• Language: English
• Subjects: biocompatibility; Biocompatible Materials; calcium phosphate; Calcium Phosphates; cell cultures; Cell Differentiation; Cell Line; Cell Proliferation; Crystallization; Culture Media; Humans; L-Lactate Dehydrogenase - metabolism; Microscopy, Electron, Scanning; Silicon; X-Ray Diffraction
• ISSN: 1549-3296; 0021-9304; 1552-4965; 1097-4636
• DOI: 10.1002/jbm.a.30790

Three silicon‐doped calcium phosphates (Si‐CaPs) were synthesized by heating precipitated silicon‐doped apatite via different thermal treatments. Temperatures of 700°C, 900°C, and 1100°C led to an apatite‐glass biphasic material, nanocrystalline Si‐doped apatite (SiHA), and Si‐doped apatite‐α tricalcium phosphate biphasic material, respectively. Structure, microstructure, textural properties, and chemical differences were determined for the three bioceramics. Biocompatibility tests were carried out by seeding osteblast‐like cells onto the three substrates. Si‐CaP treated at 700°C and 900°C led to Ca decrease in the culture media, partially impeding the cell proliferation over them. However, the proliferation capability is restored when additional culture medium is added. Finally, cytotoxicity results indicated that cell damage is much lower in osteblast‐like cells seeded onto SiHA and SiHA‐α tricalcium phosphate samples than in plastic culture control. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006