A Novel Mechanism for Complement Activation at the Surface of B Cells Following Antigen Binding

Coligation of CD21 with BCR on the surface of B cells provides a costimulatory signal essential for efficient Ab responses to T-dependent Ags. To achieve this, Ag must be directly linked to C3 fragments, but how this occurs in vivo is not fully understood. Using BCR transgenic mice, we demonstrated...

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Bibliographic Details
Published in:Journal of Immunology 2006-10, Vol.177 (8), p.5155-5162
Main Authors: Manderson, Anthony P, Quah, Ben, Botto, Marina, Goodnow, Chris C, Walport, Mark J, Parish, Chris R
Format: Article
Language:English
Subjects:
Animals
Antigen-Antibody Complex - immunology
Antigens - metabolism
B-Lymphocytes - immunology
B-Lymphocytes - physiology
Complement Activation - immunology
Complement C3 - metabolism
Immunoglobulin M - metabolism
Lymphocyte Activation - immunology
Mice
Mice, Transgenic
Receptors, Antigen, B-Cell - immunology
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Summary:Coligation of CD21 with BCR on the surface of B cells provides a costimulatory signal essential for efficient Ab responses to T-dependent Ags. To achieve this, Ag must be directly linked to C3 fragments, but how this occurs in vivo is not fully understood. Using BCR transgenic mice, we demonstrated that C3 was deposited on the surface of B cells following both high- and moderate-affinity Ag binding. This was dependent on the specific binding of IgM to the BCR-bound Ag and can occur independently of soluble immune complex formation. Based on these data, we propose a novel model in which immune complexes can form directly on the surface of the B cell following Ag binding. This model has implications for our understanding of B lymphocyte activation.
ISSN:0022-1767
1550-6606
1365-2567
DOI:10.4049/jimmunol.177.8.5155