Cloning of a d-serine-regulated transcript dsr-2 from rat cerebral neocortex

D-serine is now considered to be an endogenous co-agonist of the NMDA receptor in mammalian brain. To obtain insight into the molecular mechanisms underlying D-serine metabolism and function, we explored transcripts that are responsive to D-serine in the neocortex of the 8-day-old infant rat by a di...

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Published in:Journal of neurochemistry 2005-12, Vol.95 (6), p.1541-1549
Main Authors: TANIGUCHI, Go, YAMAMOTO, Naoki, TSUCHIDA, Hideto, UMINO, Asami, SHIMAZU, Dai, SAKURAI, Shin-Ichiro, TAKEBAYASHI, Hironao, NISHIKAWA, Toru
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - metabolism
Animals
Base Sequence
Biological and medical sciences
Blotting, Northern
Blotting, Southern
Central Nervous System - metabolism
Cloning, Molecular
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
DNA Fingerprinting
Gene Expression Regulation - physiology
Male
Medical sciences
Molecular Sequence Data
Neocortex - metabolism
Nerve Tissue Proteins - genetics
Neurology
Prosencephalon - metabolism
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
RNA - biosynthesis
Serine - physiology
Tissue Distribution
Vascular diseases and vascular malformations of the nervous system
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Summary:D-serine is now considered to be an endogenous co-agonist of the NMDA receptor in mammalian brain. To obtain insight into the molecular mechanisms underlying D-serine metabolism and function, we explored transcripts that are responsive to D-serine in the neocortex of the 8-day-old infant rat by a differential cloning technique, RNA arbitrarily primed PCR. We isolated a novel D-serine inducible transcript, D-serine-responsive transcript-2 (dsr-2), that was exclusively expressed in the brain. Sequence analysis of the corresponding cDNAs to the transcript revealed that the dsr-2 mRNA consists of 7199 nucleotides with an open reading frame encoding 111 amino acids. The dsr-2 gene was located on the reverse strand within an intron of the neurexin-3alpha gene, mapped to rat chromosome 6q24-31. The regional distribution of the basal expression of dsr-2 and its ontogenic changes in the brain closely correlated with those of free D-serine and of NMDA receptor R2B subunit mRNA, but were somewhat different from those of the neurexin-3alpha transcript. These findings suggest that dsr-2 may be involved in D-serine metabolism and/or function, and in the interactions between D-serine, NMDA receptor and neurexin-3alpha, in mammalian brain.
ISSN:0022-3042
1471-4159
DOI:10.1111/j.1471-4159.2005.03535.x