The YIG1 (YPL201c) encoded protein is involved in regulating anaerobic glycerol metabolism in Saccharomyces cerevisiae

Under anaerobic conditions S. cerevisiae produces glycerol to regenerate NAD+ from the excess NADH produced in cell metabolism. We here report on the role of an uncharacterized protein, Yig1p (Ypl201cp), in anaerobic glycerol production. Yig1p was previously shown to interact in two‐hybrid tests wit...

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Bibliographic Details
Published in:Yeast (Chichester, England) England), 2005-12, Vol.22 (16), p.1257-1268
Main Authors: Granath, K., Modig, T., Forsmark, A., Adler, L., Lidén, G.
Format: Article
Language:English
Subjects:
Anaerobiosis
Blotting, Northern
Blotting, Western
DNA, Fungal - genetics
DNA, Fungal - metabolism
Gene Expression
glycerol
Glycerol - metabolism
Glycerolphosphate Dehydrogenase - biosynthesis
Glycerolphosphate Dehydrogenase - genetics
Glycerolphosphate Dehydrogenase - metabolism
GPP1
Oxidation-Reduction
phosphatase
Phosphoric Monoester Hydrolases - biosynthesis
Phosphoric Monoester Hydrolases - genetics
Phosphoric Monoester Hydrolases - metabolism
Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
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Summary:Under anaerobic conditions S. cerevisiae produces glycerol to regenerate NAD+ from the excess NADH produced in cell metabolism. We here report on the role of an uncharacterized protein, Yig1p (Ypl201cp), in anaerobic glycerol production. Yig1p was previously shown to interact in two‐hybrid tests with the GPP1 and GPP2 encoded glycerol 3‐phosphatase (Gpp), and we here demonstrate that strains overexpressing YIG1 show strongly decreased Gpp activity and content of the major phosphatase, Gpp1p. However, cells overexpressing YIG1 exhibited only slightly decreased GPP1 transcript levels, suggesting that Yig1p modulates expression on both transcriptional and post‐transcriptional levels. In agreement with such a role, a GFP‐tagged derivate of Yig1p was localized to both the cytosol and the nucleus. Deletion or overexpression of YIG1 did not, however, significantly affect growth yield or glycerol yield in anaerobic batch cultures, which is consistent with the previously proposed low flux control exerted at the Gpp level. Copyright © 2005 John Wiley & Sons, Ltd.
ISSN:0749-503X
1097-0061
DOI:10.1002/yea.1307