Chiral separations on multichannel microfluidic chips

• Published in: Electrophoresis 2005-12, Vol.26 (24), p.4774-4779
• Main Authors: Gao, Yan, Shen, Zheng, Wang, Hui, Dai, Zhongpeng, Lin, Bingcheng
• Format: Article
• Language: English
• Subjects: Baclofen - analogs & derivatives; Baclofen - isolation & purification; beta-Cyclodextrins; Chiral selector screening; Electrophoresis, Microchip - instrumentation; Electrophoresis, Microchip - methods; Fluorescein-5-isothiocyanate - chemistry; Fluorescent Dyes - chemistry; gamma-Cyclodextrins; LIF; Microfluidic; Multichannel; Neutral CD; Octopamine - analogs & derivatives; Octopamine - isolation & purification; Stereoisomerism; Tocainide - analogs & derivatives; Tocainide - isolation & purification
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/elps.200500283

Chiral separations of FITC‐labeled basic drugs on multichannel microfluidic chips with LIF detector were investigated. A preliminary screening procedure for seven neutral CDs was performed under optimized conditions for chiral separations of three FITC‐labeled drugs (baclofen, norfenefrine, and tocainide) on a mono‐channel microfluidic chip. According to the results of screening, FITC‐baclofen and FITC‐norfenefrine as well as two chiral selectors including γ‐CD and dimethyl‐β‐CD (DM‐β‐CD) were selected as models to perform chiral separations on a two‐channel chip. FITC‐baclofen enantiomers were separated completely by γ‐CD in one channel, while resolution of FITC‐norfenefrine enantiomers was achieved by DM‐β‐CD in the other channel in the same run. Furthermore, the feasibility of using one chiral selector to separate multiple chiral samples was studied on a four‐channel chip. These results show that multichannel chip has a potential for chiral high‐throughput screening.