Rearrangement of a conditional allele regardless of inheritance of a Cre recombinase transgene

To generate conditional gene knockouts in osteoblasts, we previously developed transgenic mice in which Cre recombinase cDNA was cloned downstream of a 3.6 or 2.3 kb fragment of the rat Col1a1 promoter (Col3.6‐Cre and Col2.3‐Cre, respectively). Col‐Cre mice were bred with mice in which exon 4 of the...

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Published in:Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2007-01, Vol.45 (1), p.17-20
Main Authors: Cochrane, Rebecca L., Clark, Stephen H., Harris, Adam, Kream, Barbara E.
Format: Article
Language:English
Subjects:
Alleles
Animals
Col1a1 promoter
conditional knockout
Cre-loxP
Female
Igf1
Inheritance Patterns
Insulin-Like Growth Factor I - genetics
Insulin-Like Growth Factor I - metabolism
Integrases - genetics
Male
Mice
Mice, Transgenic
osteoblast
Transgenes
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Summary:To generate conditional gene knockouts in osteoblasts, we previously developed transgenic mice in which Cre recombinase cDNA was cloned downstream of a 3.6 or 2.3 kb fragment of the rat Col1a1 promoter (Col3.6‐Cre and Col2.3‐Cre, respectively). Col‐Cre mice were bred with mice in which exon 4 of the Igf1 gene is flanked by loxP sites. Mating units were arranged such that either the male or the female breeder transmitted the Col‐Cre transgenes. Progeny were evaluated for Cre‐mediated Igf1 gene rearrangement. We found that the loxP‐flanked Igf1 locus was rearranged in the absence of inheritance of the Cre transgene. The incidence was 50 and 28% with Col2.3‐Cre and Col3.6‐Cre females, respectively, and 15 and 18% with Col2.3‐Cre and Col3.6‐Cre males, respectively. genesis 45:17–20, 2007. © 2007 Wiley‐Liss, Inc.
ISSN:1526-954X
1526-968X
DOI:10.1002/dvg.20259