Isolation of Bunyamwera Serogroup Viruses (Bunyaviridae, Orthobunyavirus) in New York State

During routine arbovirus surveillance from 2000 to 2004 in New York state (NYS), 14,788 mosquito pools making up 36 species and nine genera were inoculated onto Vero cell cultures to test for a broad spectrum of viruses. Forty-six percent of viruses isolated in cell culture from species, excluding C...

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Bibliographic Details
Published in:Journal of medical entomology 2006-09, Vol.43 (5), p.1004-1009
Main Authors: Ngo, Kiet A., Maffei, Joseph G., Dupuis, Alan P., Kauffman, Elizabeth B., Backenson, P. Bryon, Kramer, Laura D.
Format: Article
Language:English
Subjects:
Animals
Arbovirus
arboviruses
Biological and medical sciences
Bunyamwera serogroup
Bunyamwera virus
Bunyamwera virus - genetics
Bunyamwera virus - isolation & purification
Bunyaviridae
Bunyavirus
Cache Valley virus
Cercopithecus aethiops
Culicidae
Culicidae - virology
disease detection
disease surveillance
DNA Primers
Female
Fundamental and applied biological sciences. Psychology
Geography
insect vectors
Insect Vectors - virology
isolation
Medically important nuisances and vectors, pests of stored products and materials: population survey and control
New York
pathogen identification
Potosi virus
reverse transcriptase polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction - methods
VECTOR-BORNE DISEASES, SURVEILLANCE, PREVENTION
Vectors. Intermediate hosts
Vero Cells
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Summary:During routine arbovirus surveillance from 2000 to 2004 in New York state (NYS), 14,788 mosquito pools making up 36 species and nine genera were inoculated onto Vero cell cultures to test for a broad spectrum of viruses. Forty-six percent of viruses isolated in cell culture from species, excluding Culex pipiens L. and Culex restuans Theobald, were identified as Bunyamwera serogroup viruses. Here, we report the distribution and level of Bunyamwera activity in NYS detected during this period. We developed specific primers for Cache Valley virus (family Bunyaviridae, genus Orthobunyavirus, CVV) and Potosi virus (family Bunyaviridae, genus Orthobunyavirus, POTV), to facilitate rapid molecular identification of these viruses. Viral RNA was detected in 12 mosquito species by reverse transcription-polymerase chain reaction, with the majority isolated from Aedes trivittatus (Coquillet). We report the first POTV isolation in NYS and describe the development of specific primers to identify both POTV and CVV.
ISSN:0022-2585
1938-2928
DOI:10.1603/0022-2585%282006%2943%5B1004%3AIOBSVB%5D2.0.CO%3B2