Central actions of angiotensin II on spontaneous baroreflex sensitivity in the trout Onc orhynchus mykiss

• Published in: Regulatory peptides 2007-02, Vol.138 (2), p.94-102
• Main Authors: Lancien, Frédéric, Le Mével, Jean-Claude
• Format: Article
• Language: English
• Subjects: Angiotensin II; Angiotensin II - administration & dosage; Angiotensin II - pharmacology; Animals; Atropine - administration & dosage; Atropine - pharmacology; Baroreflex; Baroreflex - drug effects; Biological and medical sciences; Blood pressure; Blood Pressure - drug effects; Bronchodilator Agents - administration & dosage; Bronchodilator Agents - pharmacology; Cardiovascular system; Dose-Response Relationship, Drug; Electrocardiography; Fish brain; Fundamental and applied biological sciences. Psychology; Heart rate; Heart Rate - drug effects; Injections, Intraventricular; Neuropeptides; Oncorhynchus mykiss - physiology; Signal processing; Time Factors; Vasoconstrictor Agents - administration & dosage; Vasoconstrictor Agents - pharmacology; Vertebrates: endocrinology
• ISSN: 0167-0115; 1873-1686
• DOI: 10.1016/j.regpep.2006.08.008

The goal of the present study was to investigate the central action of native angiotensin II (ANG II) on the spontaneous baroreflex sensitivity (BRS) in unanesthetized trout. The animals were equipped with two subcutaneous electrocardiographic (ECG) electrodes, a dorsal aorta catheter and an intracerebroventricular (ICV) cannula which was inserted within the third ventricle of the brain. The ECG and the systolic blood pressure (SBP) signals were recorded during a pre-injection period of 5 min and during five post-injection periods of 5 min. All injections were made at the fifth minute of the test. The time-series were processed with a sequence technique in order to detect the sequences of three or more consecutive increases in the SBP pulse, or three or more decreases in the SBP pulse correlated respectively with one delay beat increase of the RR interval of the ECG signal or shortening of this interval. The slope of the average regression line between the SBP and the RR intervals for each type of sequence was taken as a measure of the spontaneous BRS. Compared with pre-injection values, the ICV injection of vehicle (0.5 μl) had no effect on heart rate (HR), SBP, the total number of positive or negative sequences or on the spontaneous BRS during the post-injection periods. By contrast, ANG II at doses of 5 and 50 pmol increased HR but only 50 pmol ANG II elevated SBP. For all doses, ANG II depressed the spontaneous BRS, but the peptide had no effect upon the number of each baroreflex sequences. Intra-arterial injections of atropine dramatically reduced the number of positive and negative baroreflex sequences and decreased the sensitivity of the few remaining sequences, suggesting that the autonomic control of the cardiac BRS was solely due to vagal parasympathetic control. In atropinized trout the ICV injection of 5 pmol ANG II had no effect upon HR, SBP and the baroreflex parameters. This study determines for the first time the spontaneous BRS in a non-mammalian species and demonstrates an inhibitory action of ICV injection of ANG II upon this variable through a probable control of the vagal parasympathetic activity.