Novel gene members in the Pho regulon of Escherichia coli

Abstract The transcriptome profiles of the wild-type and the phoB mutant strains were compared at the time point showing the highest expression levels of the phoB and phoR genes under a P-limiting condition. Among the 18 new putative genes that were found to be under the control of the PhoB transcri...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 2006-11, Vol.264 (1), p.104-109
Main Authors: Hwan Baek, Jong, Yup Lee, Sang
Format: Article
Language:English
Subjects:
AMP
AMP nucleosidase
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacterial Proteins - physiology
Bacteriology
Base Sequence
Biological and medical sciences
Consensus Sequence
Conserved sequence
E coli
Escherichia coli
Escherichia coli - genetics
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Fluorescence
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation, Bacterial
Genes
Genes, Bacterial
Genes, Reporter
Genetics
Glycosyltransferases - genetics
Glycosyltransferases - metabolism
Green fluorescent protein
Green Fluorescent Proteins - analysis
Metal ions
Microbiology
Molecular and cellular biology
Molecular genetics
Mutation
N-Glycosyl Hydrolases - genetics
N-Glycosyl Hydrolases - metabolism
Pho regulon
phosphate starvation response
Proteins
Recombinant Fusion Proteins - analysis
Regulatory Elements, Transcriptional
Regulon
Reporter gene
Transcription
Transcription. Transcription factor. Splicing. Rna processing
transcriptional analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract The transcriptome profiles of the wild-type and the phoB mutant strains were compared at the time point showing the highest expression levels of the phoB and phoR genes under a P-limiting condition. Among the 18 new putative genes that were found to be under the control of the PhoB transcriptional regulator, five genes that contain the consensus Pho box were identified by sequence analysis. A reporter gene assay was carried out by fusing the upstream regions of these genes to the promoterless enhanced green fluorescent protein gene, followed by expression. It was found that the expressions of the amn (AMP nucleosidase), yibD (metal ion stress response gene) and ytfK (hypothetical protein) genes were activated by PhoB. These results indicate the additional roles of PhoB as a global regulator.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2006.00440.x