A Complete Microfluidic Screening Platform for Rational Protein Crystallization

We describe a complete microfluidic screening platform for protein crystallization that integrates fully programmable on-chip combinatorial mixing with dense assay storage using two-phase flows. This device supports rational crystallization strategies based on systematic solubility phase space mappi...

Full description

Saved in:
Bibliographic Details
Published in:Journal of the American Chemical Society 2007-01, Vol.129 (3), p.454-455
Main Authors: Lau, Billy T. C, Baitz, Chris A, Dong, Xiao P, Hansen, Carl L
Format: Article
Language:English
Subjects:
Biotechnology - methods
Crystallization - methods
Crystallography, X-Ray
Microfluidic Analytical Techniques - methods
Proteins - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We describe a complete microfluidic screening platform for protein crystallization that integrates fully programmable on-chip combinatorial mixing with dense assay storage using two-phase flows. This device supports rational crystallization strategies based on systematic solubility phase space mapping followed by nanoliter volume crystallization screening. Sequential formulation of arbitrary chemical mixtures and injection of nanoliter volume droplets into an immiscible carrier fluid creates a water/oil emulsion with independent control over droplet chemistry, size, frequency, and spacing. Long-term incubation of droplets with controlled dehydration is used to induce crystallization.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja065855b