RBMX is a novel hepatic transcriptional regulator of SREBP-1c gene response to high-fructose diet

In rodents a high-fructose diet induces metabolic derangements similar to those in metabolic syndrome. Previously we suggested that in mouse liver an unidentified nuclear protein binding to the sterol regulatory element (SRE)-binding protein-1c (SREBP-1c) promoter region plays a key role for the res...

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Bibliographic Details
Published in:FEBS letters 2007-01, Vol.581 (2), p.218-222
Main Authors: Takemoto, Tadashi, Nishio, Yoshihiko, Sekine, Osamu, Ikeuchi, Chikako, Nagai, Yoshio, Maeno, Yasuhiro, Maegawa, Hiroshi, Kimura, Hiroshi, Kashiwagi, Atsunori
Format: Article
Language:English
Subjects:
Animals
Antibodies - pharmacology
Cell Line, Tumor
Diet
Electrophoretic Mobility Shift Assay
EMSA
Fructose
Fructose - administration & dosage
heterogeneous nuclear ribonucleoprotein G
Heterogeneous-Nuclear Ribonucleoproteins - antagonists & inhibitors
Heterogeneous-Nuclear Ribonucleoproteins - genetics
Heterogeneous-Nuclear Ribonucleoproteins - metabolism
hnRNPG
Liver - metabolism
Metabolic syndrome
Mice
Mice, Inbred Strains
Promoter Regions, Genetic - drug effects
Rats
RBMX
RBMY
RNA Interference
RNA-binding motif protein on the X chromosome
RNA-binding motif protein on the Y chromosome
RNAi
siRNA
small interference RNA
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
SREBP
SREBP-1c
sterol regulatory element (SRE)-binding protein
Sterol Regulatory Element Binding Protein 1 - genetics
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Description
Summary:In rodents a high-fructose diet induces metabolic derangements similar to those in metabolic syndrome. Previously we suggested that in mouse liver an unidentified nuclear protein binding to the sterol regulatory element (SRE)-binding protein-1c (SREBP-1c) promoter region plays a key role for the response to high-fructose diet. Here, using MALDI-TOF MASS technique, we identified an X-chromosome-linked RNA binding motif protein (RBMX) as a new candidate molecule. In electrophoretic mobility shift assay, anti-RBMX antibody displaced the bands induced by fructose-feeding. Overexpression or suppression of RBMX on rat hepatoma cells regulated the SREBP-1c promoter activity. RBMX may control SREBP-1c expression in mouse liver in response to high-fructose diet.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2006.12.014