tomato pathogen Clavibacter michiganensis ssp. michiganensis: producer of several antimicrobial substances

To purify and analyse antimicrobial substances produced by the tomato pathogen Clavibacter michiganensis ssp. michiganensis (Cmm), with potential application in control of Clavibacter michiganensis ssp. sepedonicus (Cms), the causal agent of bacterial ring rot of potato. After selection of a suitabl...

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Bibliographic Details
Published in:Journal of applied microbiology 2007-02, Vol.102 (2), p.416-423
Main Authors: Holtsmark, I, Mantzilas, D, Eijsink, V.G.H, Brurberg, M.B
Format: Article
Language:English
Subjects:
Actinomycetales - metabolism
Amino Acids - analysis
Anti-Bacterial Agents - analysis
Anti-Bacterial Agents - biosynthesis
Anti-Bacterial Agents - pharmacology
antimicrobial
Bacteriocins - analysis
Bacteriocins - biosynthesis
Bacteriological Techniques
Biological and medical sciences
Chromatography, Ion Exchange
Clavibacter
Electrophoresis, Polyacrylamide Gel
Endopeptidase K - metabolism
Fundamental and applied biological sciences. Psychology
Gram-positive
lantibiotic
Lycopersicon esculentum - microbiology
Microbial Sensitivity Tests
Microbiology
Molecular Weight
Plant Diseases - microbiology
plant pathogen
plant pathogens
Solanum tuberosum - microbiology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
tunicamycin
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Summary:To purify and analyse antimicrobial substances produced by the tomato pathogen Clavibacter michiganensis ssp. michiganensis (Cmm), with potential application in control of Clavibacter michiganensis ssp. sepedonicus (Cms), the causal agent of bacterial ring rot of potato. After selection of a suitable producer and indicator strain, antimicrobial compounds were isolated using chromatographic techniques. The resulting preparations were analysed with respect to heat and protease sensitivity, amino acid composition, amino acid sequence and mass. Using this procedure we discovered one post-translationally modified 2145 Da peptide bacteriocin, one 14 kDa antimicrobial protein as well as low molecular weight (
ISSN:1364-5072
1365-2672
DOI:10.1111/j.1365-2672.2006.03091.x