Differential gene expression patterns in porcine nuclear transfer embryos reconstructed with fetal fibroblasts and mesenchymal stem cells

The present study compared the developmental ability and gene expression pattern at 4‐cell, 8‐cell, morula, and blastocyst stages of porcine nuclear transfer (NT) embryos from fetal fibroblasts (FFs) and mesenchymal stem cells (MSCs), in vitro fertilized (IVF), and in vivo derived embryos. MSC‐NT em...

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Published in:Developmental dynamics 2007-02, Vol.236 (2), p.435-446
Main Authors: Kumar, B. Mohana, Jin, Hai‐Feng, Kim, Jung‐Gon, Ock, Sun‐A, Hong, Yonggeun, Balasubramanian, S., Choe, Sang‐Yong, Rho, Gyu‐Jin
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Antigens, Surface - metabolism
Apoptosis - physiology
DNA Primers
embryo
Embryonic Development - genetics
Embryonic Development - physiology
Fertilization in Vitro
gene expression
Gene Expression Profiling
Gene Expression Regulation, Developmental
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
nuclear transfer
Nuclear Transfer Techniques
porcine
Reverse Transcriptase Polymerase Chain Reaction
Sus scrofa
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Summary:The present study compared the developmental ability and gene expression pattern at 4‐cell, 8‐cell, morula, and blastocyst stages of porcine nuclear transfer (NT) embryos from fetal fibroblasts (FFs) and mesenchymal stem cells (MSCs), in vitro fertilized (IVF), and in vivo derived embryos. MSC‐NT embryos showed enhanced blastocyst formation, higher total cell number, and a low incidence of apoptosis compared to FF‐NT embryos. Alterations in the expression pattern of genes implicated in transcription and pluripotency (Oct4, Stat3, Nanog), DNA methylation (Dnmt1, Dnmt3a), histone deacetylation (Hdac2), growth factor signaling, and imprinting (Igf2, Igf2r) and apoptosis (Bax, Bcl2) regulation were observed in NT embryos. The expression of transcripts in MSC‐NT embryos more closely followed that of the in vivo derived embryos compared with FF‐NT embryos. In conclusion, MSCs with a relatively undifferentiated genome might serve as suitable donors that could be more efficiently reprogrammed to re‐activate expression of early embryonic genes in porcine NT. Developmental Dynamics 236:435–446, 2007. © 2006 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.21042