Genotypic characterisation of Indian cattle, buffalo and sheep isolates of Echinococcus granulosus

Twelve isolates of Echinococcus granulosus, collected from domestic animals, including cattle, buffalo and sheep were analysed for DNA nucleotide sequence variation within mitochondrial cytochrome c oxidase I ( coxI), NADH dehydrogenase subunit I ( nadI) and internal transcribed spacer gene I (ITS1)...

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Bibliographic Details
Published in:Veterinary parasitology 2007-02, Vol.143 (3), p.371-374
Main Authors: Bhattacharya, D., Bera, A.K., Bera, B.C., Maity, A., Das, S.K.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Buffalo
buffaloes
Buffaloes - parasitology
Cattle
Cattle Diseases - parasitology
cytochrome-c oxidase
Cytochromes c1 - genetics
DNA, Helminth - analysis
DNA, Helminth - chemistry
DNA, Intergenic - genetics
echinococcosis
Echinococcosis - parasitology
Echinococcosis - veterinary
Echinococcus granulosus
Echinococcus granulosus - classification
Echinococcus granulosus - genetics
Echinococcus granulosus - isolation & purification
Genetic Markers
genetic variation
Genotype
Genotypes
internal transcribed spacers
mitochondrial DNA
Molecular Sequence Data
NADH dehydrogenase
NADH Dehydrogenase - genetics
nucleotide sequences
Phylogeny
Sequence Homology
Sheep
Sheep Diseases - parasitology
strain differences
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Description
Summary:Twelve isolates of Echinococcus granulosus, collected from domestic animals, including cattle, buffalo and sheep were analysed for DNA nucleotide sequence variation within mitochondrial cytochrome c oxidase I ( coxI), NADH dehydrogenase subunit I ( nadI) and internal transcribed spacer gene I (ITS1). After analysis of sequence information this was found that the fragment size of ITS1 of buffalo isolate was more in comparison to cattle and sheep isolates. Based on the nadI genotype this was found that Indian cattle, buffalo and sheep isolates could be grouped into E. granulosus sensu stricto. Based on coxI genotype two sheep isolates and one buffalo isolate were homologous to G2 genotype. Rests of the isolates were microvariants of G2 genotype. Presence of G2 genotype in buffalo is the first report of this genotype from this host.
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2006.09.007