Protein Biomarkers in a Mouse Model of Extremes in Trait Anxiety

Brain proteome analysis of mice selectively bred for either high or low anxiety-related behavior revealed quantitative and qualitative protein expression differences. The enzyme glyoxalase-I was consistently expressed to a higher extent in low anxiety as compared with high anxiety mice in several br...

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Bibliographic Details
Published in:Molecular & cellular proteomics 2006-10, Vol.5 (10), p.1914-1920
Main Authors: Ditzen, Claudia, Jastorff, Archana M., Kessler, Melanie S., Bunck, Mirjam, Teplytska, Larysa, Erhardt, Angelika, Krömer, Simone A., Varadarajulu, Jeeva, Targosz, Bianca-Sabrina, Sayan-Ayata, Eser F., Holsboer, Florian, Landgraf, Rainer, Turck, Christoph W.
Format: Article
Language:English
Subjects:
Amygdala - chemistry
Amygdala - metabolism
Animals
Anxiety - chemically induced
Anxiety - diagnosis
Anxiety - metabolism
Behavior, Animal
Biomarkers - analysis
Biomarkers - metabolism
Blood Proteins - analysis
Blood Proteins - metabolism
Blotting, Western
Disease Models, Animal
Electrophoresis, Gel, Two-Dimensional
Female
Humans
Lactoylglutathione Lyase - metabolism
Male
Mice
Phosphopyruvate Hydratase - metabolism
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Summary:Brain proteome analysis of mice selectively bred for either high or low anxiety-related behavior revealed quantitative and qualitative protein expression differences. The enzyme glyoxalase-I was consistently expressed to a higher extent in low anxiety as compared with high anxiety mice in several brain areas. The same phenotype-dependent difference was also found in red blood cells with normal and cross-mated animals showing intermediate expression profiles of glyoxalase-I. Another protein that showed a different mobility during two-dimensional gel electrophoresis was identified as enolase phosphatase. The presence of both protein markers in red or white blood cells, respectively, creates the opportunity to screen for their expression in clinical blood specimens from patients suffering from anxiety.
ISSN:1535-9476
1535-9484
DOI:10.1074/mcp.M600088-MCP200