Analysis of prominent flavonoid aglycones by high-performance liquid chromatography using a monolithic type column

• Published in: Journal of Chromatography A 2006-10, Vol.1131 (1), p.51-57
• Main Authors: REPOLLES, Carme, HERRERO-MARTINEZ, José Manuel, RAFOLS, Clara
• Format: Article
• Language: English
• Subjects: Acetonitriles - chemistry; Analytical chemistry; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Exact sciences and technology; Flavonoids; Flavonoids - analysis; Flavonoids - chemistry; Food Analysis; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; General pharmacology; Ginkgo biloba - chemistry; Medical sciences; Methanol - chemistry; Methods of analysis, processing and quality control, regulation, standards; Molecular Structure; Monolithic columns; Other chromatographic methods; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Reproducibility of Results
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2006.07.012

A fast RP-HPLC method for the simultaneous separation of eleven main flavonoid aglycones in food samples was developed. Flavonoids were divided in three groups according to their hydrophobicity and were resolved by a ternary gradient elution and diode array detection within analysis times less than 15 min. The detection limits of the flavonoids studied were in the range between 0.04 and 0.31 μg/mL, with repeatabilities values between 0.4 and 5%. The method was successfully applied to the analysis of these compounds in complex natural samples, like propolis and Ginkgo biloba.